Up-regulation of hnRNP A1, Ezrin, tubulin β-2C and Annexin A1 in sentinel lymph nodes of colorectal cancer  

作　　者：Zhen-Yu He  Hao Wen Zhen-Yu He  Jie Wang  Chuan-Bing Shi  Jie Wang[4] 

机构地区：[1]Department of General Surgery Second Affiliated Hospital of Nanjing Medical University 121 Jiangjiayuan Road Nanjing 210011 Jiangsu Province China [2] First Clinic College of Nanjing Medical University Nanjing 210029 China [3]Depa rtment of Pathology,Second Affiliated Hospital of Nanjing Medical University,121 Jiangjiayuan Road,Nanjing 210011,Jiangsu Province,China [4]Department of Interventional Radiology,First Af��liated Hospital and First Clinic College of Nanjing Medical University, 300 Guangzhou Road,Nanjing 210029,Jiangsu Province,China

出　　处：《World Journal of Gastroenterology》2010年第37期4670-4676,共7页世界胃肠病学杂志（英文版）

基　　金：Supported by The Natural Science Basic Research Project,Education Department of Jiangsu Province, No. 08KJT310005;the 5th "Six Talent-Person-Peak Program", Jiangsu Province,China;Superior Item of Nanjing Medical University Science and Technology Progress Fund, No. 07NMUM047

摘　　要：AIM: To investigate the early metastasis-associated proteins in sentinel lymph node micrometastasis (SLNMM) of colorectal cancer (CRC) through comparative proteome. METHODS: Hydrophobic protein samples were extracted from individual-matched normal lymph nodes (NLN) and SLNMM of CRC. Differentially expressed protein spots were detected by two-dimensional electrophoresis and image analysis, and subsequently identified by matrix assisted laser desorption/ionization-time of flight mass spectrometry-mass spectrometry and Western blotting, respectively.RESULTS: Forty proteins were differentially expressed in NLN and SLNMM, and 4 metastasis-concerned proteins highly expressed in SLNMM were identified to be hnRNP A1, Ezrin, tubulin β-2C and Annexin A1. Further immunohistochemistry staining of these four proteins showed their clinicopathological characteristics in lymph node metastasis of CRC. CONCLUSION: Variations of hydrophobic protein expression in NLN and SLNMM of CRC and increased expression of hnRNP A1, Ezrin, tubulin β-2C and Annexin A1 in SLNMM suggest a significantly elevated early CRC metastasis.AIM: To investigate the early metastasis-associated proteins in sentinel lymph node micrometastasis (SLNMM) of colorectal cancer (CRC) through comparative proteome. METHODS: Hydrophobic protein samples were extracted from individual-matched normal lymph nodes (NLN) and SLNMM of CRC. Differentially expressed protein spots were detected by two-dimensional electrophoresis and image analysis, and subsequently identified by matrix assisted laser desorption/ionization-time of flight mass spectrometry-mass spectrometry and Western blotting, respectively.RESULTS: Forty proteins were differentially expressed in NLN and SLNMM, and 4 metastasis-concerned proteins highly expressed in SLNMM were identified to be hnRNP A1, Ezrin, tubulin β-2C and Annexin A1. Further immunohistochemistry staining of these four proteins showed their clinicopathological characteristics in lymph node metastasis of CRC. CONCLUSION: Variations of hydrophobic protein expression in NLN and SLNMM of CRC and increased expression of hnRNP A1, Ezrin, tubulin β-2C and Annexin A1 in SLNMM suggest a significantly elevated early CRC metastasis.

关 键 词：Colorectal cancer MICROMETASTASIS PROTEOMICS Sentinel lymph node 

分 类 号：R735.34[医药卫生—肿瘤]