辣椒素对肝癌HepG2细胞凋亡的诱导作用  

Induction of Capsaicin on Apoptosis of Human Hepatoma HepG2 Cells

作　　者：严斌[1] 杜春玲[2] 李艳兵[1] 曾少波[1] 江斌[1] 

机构地区：[1]湖北医药学院附属太和医院肝胆外科,湖北十堰442000 [2]湖北医药学院附属太和医院呼吸内科,湖北十堰442000

出　　处：《郧阳医学院学报》2010年第6期509-511,609,共4页Journal of Yunyang Medical College

摘　　要：目的:探讨辣椒素对肝癌HepG2细胞生长抑制及凋亡诱导作用的机制。方法:以100、200、400μmol/L浓度辣椒素处理肝癌HepG2细胞后分别培养12、24、48 h,MTT法测定细胞增殖抑制率;TUNEL法检测细胞凋亡;Western Blotting检测凋亡相关蛋白Bcl-2、Bax、Bad的表达。结果:随着作用浓度及时间增加,经辣椒素处理的肝癌HepG2细胞增殖活性明显降低(P<0.01);抑制率均显著增加,呈浓度和时间依赖性(P<0.01);HepG2细胞凋亡率也随辣椒素浓度增加而上升;凋亡相关蛋白Bcl-2表达下降,Bax、Bad表达上升(P<0.05)。结论:辣椒素对肝癌HepG2细胞具有增殖抑制和诱导凋亡作用,其机制可能与调节凋亡相关蛋白有关。Objective To investigate the inductive effect of capsaicin on human hepatoma HepG2 cells apoptosis and its possible mechanism.Methods HepG2 cells were treated with various concentrations of capsaicin,and the inhibitory rate of cell proliferation was measured by MTT at different time points,the apoptosis of cells were determined by TUNEL assay,the expression of Bcl-2,Bax and Bad proteins were detected by Western blotting.Results The proliferation of HepG2 cells was significantly inhibited following the increasing concentration and time lasting of capsaicin.The rate of apoptosis was significantly increased with a dose-dependent manner.The expression of Bax,Bad was up-regulated,but the expression of Bcl-2 was down-regulated.Conclusion Capsaicin could inhibit the proliferation and induce the apoptosis of human hepatoma HepG2 cells,which may relate to the regulation of Bcl-2,Bax,Bad protein expression.

关 键 词：辣椒素 凋亡 HEPG2细胞 

分 类 号：R735.7[医药卫生—肿瘤]