5-脂氧酶参与鱼藤酮诱导的PC12细胞损伤  

5-lipoxygenase is involved in rotenone-induced injury in PC12 cells

作　　者：张晓燕[1] 张丽慧[1] 李成檀[1] 陈文健[1] 赵建波[1] 魏尔清[2] 

机构地区：[1]杭州师范大学医学神经生物学市级重点实验室基础医学部药理学教研室,浙江杭州310036 [2]浙江大学医学院药理学系,浙江杭州310058

出　　处：《浙江大学学报（医学版）》2011年第2期150-155,168,共7页Journal of Zhejiang University(Medical Sciences)

基　　金：杭州市重点实验室项目(20090233T12);浙江省教育厅科研基金资助项目(Y200907625);杭州师范大学国家项目培育基金(2010PYjj41)

摘　　要：目的:以鱼藤酮诱导PC12细胞损伤作为PD细胞模型,观察5-脂氧酶(5-lipoxygenase,5-LOX)是否参与细胞损伤。方法:在鱼藤酮处理后不同时间,以MTT方法检测PC12细胞活性变化,以免疫细胞化学方法观察5-LOX核膜移位情况;并观察5-LOX抑制剂齐留通的作用。结果:鱼藤酮(0.3～30μmol/L)可诱导PC12细胞损伤;齐留通(3～100μmol/L)减轻鱼藤酮诱导的细胞损伤。鱼藤酮还时间和浓度依赖性引起5-LOX核膜移位,齐留通(1～30μmo/L)则能抑制5-LOX核膜移位。结论:5-LOX参与鱼藤酮诱导的PC12细胞损伤,5-LOX抑制剂齐留通可抑制5-LOX激活,减轻细胞损伤。Objective: To determine whether 5-lipoxygenase(5-LOX)is involved in rotenone-induced injury in PC12 cells,which is a cell model of Parkinson disease.Methods: After rotenone treatment for various durations,cell viability was determined by colorimetric MTT reduction assay,and 5-LOX translocation was detected by immunocytochemistry.The effect of 5-LOX inhibitor zileuton was also investigated.Results: Rotenone(0.3-30 μmol/L) induced PC12 cell injury,and zileuton(3-100 μmol/L) attenuated this injury.Rotenone also time-and concentration-dependently induced 5-LOX translocation into the nuclear envelope,and zileuton(1-30 μmo/L) significantly inhibited rotenone-induced 5-LOX translocation.Conclusion: 5-LOX is involved in rotenone-induced injury in PC12 cells,and 5-LOX inhibitor zileuton can reduce rotenone-induced 5-LOX activation and cell injury.

关 键 词：脂氧合酶 5-脂氧酶 帕金森病 鱼藤酮/药理学 PC12细胞/药物作用 

分 类 号：R742.5[医药卫生—神经病学与精神病学]