肿瘤坏死因子α基因-238G>A及-857C>T多态性与河南汉族群体原发性高血压  

Correlation Between TNF α-238G>A and-857C>T Gene Polymorphisms and Essential Hypertension in Henan Han Population

作　　者：郭利伟[1] 杨彩玲[2] 黄艳梅[1] 李端[3] 祁英杰[1] 韩金红[1] 杨保胜[1] 

机构地区：[1]新乡医学院基础医学院,河南新乡453003 [2]新乡医学院第一附属医院,河南新乡453003 [3]新乡医学院生命科学技术系,河南新乡453003

出　　处：《河南师范大学学报（自然科学版）》2011年第3期126-130,共5页Journal of Henan Normal University(Natural Science Edition)

基　　金：河南省教育厅自然科学基金项目(2008A310010)

摘　　要：目的:探讨肿瘤坏死因子α(TNF-α)基因-238G>A及-857C>T多态性与河南汉族原发性高血压(EH)的相关性.方法:ELISA法检测260例高血压患者和394例健康对照者血浆TNF-α水平,采用限制性扩增片段长度多态性(PCR-RFLP)的方法检测TNF-α基因-238G>A及-857C>T多态性.结果:与正常对照组相比,高血压患者血浆TNF-α水平显著增高(P<0.01);EH组中TNF-α-238G>AGA+AA基因型者的DBP、TC、TG、及LDL含量明显高于GG纯合子者,差异有统计学意义(P<0.05),但是EH组TNF-α基因-238G>A及-857C>T各基因型及等位基因频率与正常对照组比较差异无统计学意义(P>0.05).结论:TNF-α-238G>AA等位基因可能会增加高血压的发病风险,但TNF-α基因-238G>A及-857C>T基因多态性与河南汉族原发性高血压无关联.Objective:To investigate the correlation between tumor necrosis factor α gene polymorphisms and Henan Han essential hypertension(EH).Methods:The levels of TNF-α in plasma is detected by ELISA.The gene polymorphisms of TNF-α-238G>A and-857C>T in 260 EH patients(EH group) and 394 healthy population(control group) are analyzed by polymerase chain reaction and restriction endonuclease technology(PCR-RFLP).Results:In EH group,the DBP,TC,TG,and LDL levels of the TNF-α-238G> A GA + AA genotype people are significantly higher than that of GG genotype(P<0.05).However,there is no significant difference in genotype and allelic frequency of the-238G>A and-857C>T polymorphism between healthy subjects and patients with EH(P>0.05).Conclusion :TNF-α-238G> A A allele might increase the risk of etiology in EH,but the gene polymorphism of TNF-α-238G>A and TNF-α-857C>T do not to be associated with EH in Henan Han population.

关 键 词：肿瘤坏死因子-Α 原发性高血压 基因多态性 

分 类 号：N55[自然科学总论] G658.3[文化科学—教育学]