过瘤胃保护性精氨酸和大豆油对细毛羊肌内脂肪和脂肪酸组成的影响  

Effects of Rumen-Protected Arginine and Soybean Oil on Intramuscular Fat Content and Fatty Acid Composition in Fine Wool Sheep

作　　者：李金霞 孙海洲[2] 赵存发[3] 桑丹[2] 张春华[2] 凌树礼[2] 珊丹[2] 任晓萍[2] 

机构地区：[1]内蒙古海拉尔农垦特泥河分公司,海拉尔021024 [2]内蒙古农牧业科学院动物营养与饲料研究所,呼和浩特010030 [3]内蒙古农牧业科学院,呼和浩特010030

出　　处：《动物营养学报》2012年第5期828-834,共7页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家绒毛用羊产业技术体系建设(nycytx-40);国家农业科技成果转化资金项目(2009A40020);内蒙古农牧业科技创新基金项目(2008CXJJM01)

摘　　要：本试验旨在研究饲粮添加不同水平的过瘤胃保护性精氨酸(rumen-protected arginine,RP-Arg)和大豆油对细毛羊肌内脂肪和脂肪酸组成的影响。试验选用健康、体重相近的15只细毛羊,随机分为5组,对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮中添加1.5 g/d RP-Arg、2.0 g/d RP-Arg、1.5 g/d RP-Arg+3%大豆油和2.0 g/d RP-Arg+3%大豆油的饲粮。预试期为5 d,正试期为45 d。试验结束后每组选2只羊进行屠宰,取背最长肌样用于测定肉质指标和脂肪酸组成。结果表明:试验组肌肉pH、系水率和熟肉率与对照组差异不显著(P>0.05),而肌内脂肪含量显著高于对照组(P<0.05);试验组肌肉多不饱和脂肪酸含量与对照组相比显著提高(P<0.05);添加RP-Arg和大豆油有提高肌肉过氧化物酶体增生物激活受体γ(PPARγ)、脂蛋白脂酶(LPL)mRNA表达量,降低激素敏感性甘油三酯脂肪酶(HSL)mRNA表达量的趋势。综合指标,以1.5 g/d RP-Arg组与1.5 g/d RP-Arg+3%大豆油组添加效果较好。This experiment was conducted to study the effects of the different levels of dietary rumen-protected arginine(RP-Arg) and soybean oil on the intramuscular fat content and fatty acid composition in fine wool sheep.Fifteen healthy fine wool sheep with similar body weight were randomly assigned into five groups.Sheep in the control group were fed a basal diet,and sheep in the experiment groups were fed diets supplemented with 1.5 g/d RP-Arg,2.0 g/d RP-Arg,1.5 g/d RP-Arg+3% soybean oil and 2.0 g/d RP-Arg+3% soybean oil,respectively.An adaptation period lasted for 5 days and was followed by an experiment period for 45 days.At the end of the experiment,two sheep in every group were slaughtered,and longissimus dorsi muscle tissue samples were collected for determination of meat quality indices and fatty acid composition.The results showed that the RP-Arg and soybean oil supplementation significantly increased the intramuscular fat content(P<0.05),but didn't significantly affect other meat quality indices such as pH,holding water rate and cooked meat percentage(P>0.05).Polyunsaturated fatty acid content in the experiment groups was significantly higher than that in the control group(P<0.05).Furthermore,dietary RP-Arg and soybean oil had a trend to decrease the mRNA expression of hormone-sensitive lipase(HSL),while increase that of peroxisome proliferator-activated receptor-γ(PPARγ) and lipoprotein lipase(LPL) in longissimus dorsi muscle.In conclusion,the 1.5 g/d RP-Arg supplementation group and the 1.5 g/d RP-Arg+3% soybean oil supplementation group can get the better effects.

关 键 词：细毛羊 瘤胃保护性精氨酸 肌内脂肪 脂肪酸组成 

分 类 号：S82[农业科学—畜牧学]