黑暗链霉菌aprF-G基因功能的研究  

Study on Gene Functions of aprF-G in Streptomyces tenebrarius

作　　者：朱碧银[1] 洪文荣[1] 李辉[1] 

机构地区：[1]福州大学生物科学与工程学院,福建福州350108

出　　处：《食品与生物技术学报》2012年第4期391-395,共5页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(31070093);国家"十二五"科技攻关重大专项资助项目(2012ZX09201-101-008);福建省教育厅科研项目(2007F5070)

摘　　要：以研究黑暗链霉菌Tt-49中生物合成基因aprF-G生理功能为目标,利用接合转移法,将同源重组质粒pFD10导入黑暗链霉菌Tt-49,最终获得同源双交换菌株Tt-49G53,并对其生理特性进行考察。结果显示:其生长速度明显比亲株慢,产抗水平比亲株低100倍,仅为13U/mL。采用薄层层析法对其代谢产物进行分析,未检测到安普霉素。In order to block biosynthesis genes aprF and aprG of apramycin in Streptomyces tenebrarius Tt-49,recombinant plasmid pFD10 was introduced to S.tenebrarius Tt-49 by conjugation.And double crossover mutant Tt-49 G53 was obtained finally.Preliminary research on physiological characteristics for double crossover mutant Tt-49 G53 was conducted.The results showed that double crossover mutant Tt-49G53 grew more slowly than parent strain,the level of antibiotic production was 13 u/mL,which was 100 times lower than the level of parent strain.The metabolic products were analyzed by TLC.A pre-judgment of the result was that double crossover mutant Tt-49 G53 couldn't produce apramycin.It was supposed that aprF and aprG were not closely related to secondary metabolism of S.tenebrarius only,but also was associated with the growth and development of producing strain.

关 键 词：黑暗链霉菌 基因重组 分子遗传工程 

分 类 号：Q93[生物学—微生物学]