基于与α-丙氨酸/天冬酰胺美拉德反应的低聚壳聚糖衍生物的抗氧化性能研究  

The Antioxidant Activity of the Chitosan Oligosaccharide Derivatives by Maillard Reaction Between Chitosan Oligosaccharide and α-alanine/Asparagine

作　　者：孙涛[1,2] 朱云[1] 谢晶[1] 薛斌[1] 周冬香[1] 

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海海洋大学海洋科学研究院,上海201306

出　　处：《食品与生物技术学报》2012年第5期531-536,共6页Journal of Food Science and Biotechnology

基　　金：上海市生物医药和农业科技领域重点科技项目(08391911500);2009年上海市优秀学科带头人计划(09XD1402000);上海市教委重点学科建设项目(J50704)

摘　　要：研究低聚壳聚糖(COS)与α-丙氨酸/天冬酰胺(低聚壳聚糖的羰基与氨基的物质量比均为4:1)的美拉德反应。醇沉法提取与α-丙氨酸/天冬酰胺反应8、16h以及24h的低聚壳聚糖衍生物,分别记为:CA-8、CA-16、CA-24、CN-8、CN-16以及CN-24。对衍生物进行红外表征,并研究其对超氧阳离子O2.-、DPPH自由基的清除能力以及还原能力。结果显示:美拉德反应后低聚壳聚糖衍生物抗氧化能力得到显著提高。抗氧化能力强弱次序为CA-8>CN-8、CA-16>CN-16、CA-24>CN-24,即随着反应时间增加,低聚壳聚糖与α-丙氨酸美拉德反应的衍生物抗氧化性始终更强,表明与小分子氨基酸进行美拉德反应制得的壳聚糖衍生物具有更好的抗氧化性。In this study,six kinds of the chitosan oligosaccharide derivatives,named CA-8,CA-16,CA-24,CN-8,CN-16 and CN-24were prepared though Maillard reaction by heating chitosan oligosaccharide and α-alanine/ asparagine(the ratio of carbonyl group and amino group was 4:1) at different time.Then,the antioxidant activity of those derivatives was investigated with superoxide anion radical/1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging and reducing power and index.The results indicated that the chitosan oligosaccharide derivatives exhibited more strong antioxidant activity than that of chitosan oligosaccharide.Furthermore,the derivatives from chitosan oligosaccharide with α-alanine had the better antioxidant activity than that of the derivatives prepared by chitosan oligosaccharide and asparagine.The reason is that the derivatives prepared by chitosan oligosaccharide and amino acid with the smaller molecule had the better antioxidant activity.

关 键 词：低聚壳聚糖 Α-丙氨酸 天冬酰胺 美拉德反应 抗氧化性能 

分 类 号：TS201.2[轻工技术与工程—食品科学]