Interaction between fragile histamine triad and protein kinase C alpha in human non-small cell lung cancer tissues  

作　　者：Peng-hui Zhuang1, Zhao-hui Liu2, Xiao-gang Jiang3, Cheng-en Pan41. Department of Thoracic Surgery, the First Affiliated Hospital, Medical School of Xi’an Jiaotong University 2. Department of Anatomy and Histology & Embryology, Medical School of Xi’an Jiaotong University 3. Department of Genetics and Molecular Biology, Medical School of Xi’an Jiaotong University 4. Department of Geratic Surgery, the First Affiliated Hospital, Medical School of Xi’an Jiaotong University, Xi’an 710061, China. 

出　　处：《Journal of Pharmaceutical Analysis》2009年第1期57-61,共5页药物分析学报（英文版）

基　　金：supported by the Scientific and Technological Development Research Project of Shaanxi Province (No.2005k09-G1)

摘　　要：Objective To investigate the interaction between fragile histamine triad (FHIT) and protein kinase C alpha (PKCα) in human non-small cell lung cancer tissues. Methods FHIT and PKCα double positive samples were screened by immunohistochemical staining from 13 human non-small cell lung cancer tissues. Co-immunoprecipitation was performed by using anti-FHIT and anti-PKCα. The immune precipitate was analyzed by SDS-PAGE and Western blot. Results Immune precipitate staining detection showed that 3 samples out of the 13 cases were double positive for FHIT and PKCα. FHIT protein was present in the immune precipitate of anti-PKCα while there was PKCα in the immune precipitate of anti-FHITmAb. Conclusion FHIT and PKCα exist as a complex in human non-small cell lung cancer tissues, which will provide a new route for studying the pathogenesis and immunotherapy of human non-small cell lung cancer.Objective To investigate the interaction between fragile histamine triad (FHIT) and protein kinase C alpha (PKCα) in human non-small cell lung cancer tissues. Methods FHIT and PKCα double positive samples were screened by immunohistochemical staining from 13 human non-small cell lung cancer tissues. Co-immunoprecipitation was performed by using anti-FHIT and anti-PKCα. The immune precipitate was analyzed by SDS-PAGE and Western blot. Results Immune precipitate staining detection showed that 3 samples out of the 13 cases were double positive for FHIT and PKCα. FHIT protein was present in the immune precipitate of anti-PKCα while there was PKCα in the immune precipitate of anti-FHITmAb. Conclusion FHIT and PKCα exist as a complex in human non-small cell lung cancer tissues, which will provide a new route for studying the pathogenesis and immunotherapy of human non-small cell lung cancer.

关 键 词：fragile histamine triad (FHIT) protein kinase C alpha (PKCα) non-small cell lung cancer (NSCLC) CO-IMMUNOPRECIPITATION 

分 类 号：R734.2[医药卫生—肿瘤]