泡沫分离发酵液中乳链菌肽的工艺和活性研究  

A study on the process and activation of recovering nisin from the fermentation broth by foam separation

作　　者：刘云菲[1] 胡滨[1] 吴兆亮[1] 

机构地区：[1]河北工业大学化工学院,天津300130

出　　处：《河北工业大学学报》2012年第5期55-60,共6页Journal of Hebei University of Technology

摘　　要：乳链菌肽(nisin)是一种用途广泛但容易失活的抗菌肽,分离纯化难度大.本文以发酵液为实验物系,利用泡沫分离技术从中分离纯化nisin,研究了气速、温度、分布器孔径对实验物系的影响.实验结果表明,随着气速的增加,泡沫分离乳链菌肽的富集比减小,回收率增大,nisin的失活率逐渐减小;随着温度的升高,泡沫分离乳链菌肽的富集比增加,回收率降低,nisin的失活率增大;随分布器孔径增大,气泡变大,泡沫分离乳链菌肽的富集比增大,回收率降低,nisin的失活率增大.Nisin is a kind of antimicrobial peptides widely used but easy to lose activation,and it is difficult to separate and purify.In this paper,the foam separation technology is used for the recovering of nisin from the fermentation broth.The effect of the gas velocity,the temperature and the gas-distribution aperture to enrichment ratio,recovery rate and inactivation rate of nisin is discussed.The experimental results show that with the gas velocity increasing,the recovery rate increases,and the enrichment ratio and the nisin inactivation rate decrease;with the temperature increasing,the enrichment ratio and the nisin inactivation rate increase,and the recovery rate decreases;with the gas-distributor aperture augments,bubbles become larger,the enrichment ratio and the nisin inactivation rate increase,and the recovery rate decreases.

关 键 词：乳链菌肽 泡沫分离 气速 温度 分布器孔径 

分 类 号：TQ936.16[化学工程]