α-L-鼠李糖苷酶发酵过程放大研究——从5L到30L发酵罐  

Amplification of the fermentation process for α-L-rhamnosidase——from 5 L to 30 L fermenter

作　　者：张雪铭 马骏 魏春[3] 应向贤[3] 汪钊[3] 

机构地区：[1]浙江省天正设计工程有限公司,浙江杭州310012 [2]浙江海正药业股份有限公司,浙江台州318000 [3]浙江工业大学生物与环境工程学院,浙江杭州310014

出　　处：《发酵科技通讯》2013年第4期5-8,共4页Bulletin of Fermentation Science and Technology

基　　金：科技部科技型中小企业技术创新基金(12C26213302753);浙江省自然科学基金(LY12B06010)

摘　　要：以产α-L-鼠李糖苷酶(α-L-1,2-鼠李糖苷酶和α-L-1,6-鼠李糖苷酶)的黑曲霉Aspergillus niger WZ001为考察对象,研究了从5 L发酵罐到30 L发酵罐的通气量和搅拌转速的放大工艺。通气量按三种准则、搅拌转速按两种准则放大。通过实验验证,优选得到最佳放大准则为:通气量按1.5倍空气表观线速度进行放大、搅拌转速按搅拌桨叶尖线速度放大。在该优化的放大工艺条件下,30 L发酵罐中α-L-1,2-鼠李糖苷酶和α-L-1,6-鼠李糖苷酶的产量分别为2 515 U/mL和3 612 U/mL,达到5 L发酵罐的水平。To produce α-L- rhamnosidase(α-L-1, 2- rhamnosidase and α-L-1, 6- rhamnosidase)Aspergillus niger WZ001 was used to investigate the amplification technology on aeration rate and agitation speed from 5 L to 30 L fermenter. Aeration rate was amplified by three kinds of standards and agitation speed was amplified by two kinds of standards. By the experiments verification, we obtained the best amplification criteria: aeration rate was amplified with 1.5 times the apparent linear speed and agitation speed was amplified with blade tip speed. Under the optimized conditions of amplification technology, the yields of α-L-1, 2-rhamnosidase and α-L-1, 6-rhamnosidase were 2515U/mL and 3612 U/mL in 30 L fermentor, respectively, a level equal to that in 5 L fermenter.

关 键 词：黑曲霉 α-L-鼠李糖苷酶 发酵放大 搅拌桨 

分 类 号：TQ0[化学工程] TQ4