顺铂诱导人宫颈癌Hela细胞凋亡与自噬的研究  

Cisplatin induced apoptosis and autophagy on human cervical carcinoma HeLa cells

作　　者：金镇勋[1] 兰汝春[1] 王菲[1] 董长松[1] 徐冶[2] 

机构地区：[1]吉林医药学院附属医院消化科,吉林吉林132013 [2]吉林医药学院医学科研实验室,吉林吉林132013

出　　处：《吉林医药学院学报》2013年第6期401-404,共4页Journal of Jilin Medical University

基　　金：吉林省科技厅资助项目(201015240);吉林省教育厅资助项目(2013361)

摘　　要：目的探讨顺铂对Hela细胞凋亡和自噬的影响。方法用四甲基偶氮唑蓝比色法(MTT)观察顺铂对HeLa细胞生长抑制情况;Hoechst 33342荧光染色检测细胞凋亡;间接免疫荧光法检测自噬标志蛋白LC3和P62的变化;免疫印记法检测细胞凋亡蛋白Bax/Bcl-2、Caspase-3和自噬标志蛋白LC3和P62的变化。结果顺铂对Hela细胞生长抑制作用表现为时间剂量依赖关系;顺铂作用Hela细胞导致Bax/Bcl-2比值和Caspase-3表达增加,同时自噬标志蛋白LC3和P62活化。结论顺铂诱导Hela细胞发生凋亡,伴有自噬发生。Objective To investigate the apoptosis and autophagy inducing effect of Cisplatin on HeLa cells.Methods MTT assay was employed to examine the growth suppression of the cells.Hoechst33342 staining was used to observe the apoptosis.Indirect immunofluorescence was used to detect the expressions and location of the LC3 and P62 proteins.Western Blotting was used to detect the expressions of Bax/Bcl-2,Caspase-3,LC3 and P62.Results The inhibitory effect of Cisplatin was in a time and dosage dependant manner.Cisplatin could increase the ratio of Bax/Bcl-2 and expression of caspase-3.Cispaltin treatment induced activation of LC3 and P62.Conclusion Cisplatin could induce apoptosis of HeLa cells paralleled autophagy.

关 键 词：顺铂 宫颈癌 凋亡 自噬 

分 类 号：R737.33[医药卫生—肿瘤]