液相色谱-串联质谱法检测动物组织中阿布拉霉素  

Determination of apramycin residues in animal tissues by high pressure liquid chromatography-tandem mass spectrometry

作　　者：赵凤娟[1] 岳振峰[1] 肖陈贵[1] 叶刚[1] 吴卫东[1] 胡晓苑[1] 

机构地区：[1]深圳出入境检验检疫局食品检验检疫技术中心,深圳市食品安全检测技术研发重点实验室,深圳518045

出　　处：《食品安全质量检测学报》2013年第1期159-164,共6页Journal of Food Safety and Quality

摘　　要：目的建立动物源食品中阿布拉霉素的LC-MS/MS检测方法.方法待测样品中的阿布拉霉素用5%的三氯乙酸溶液提取,经MCX固相萃取柱净化、浓缩、定容后采用液相色谱-串联质谱法在正离子模式下检测.结果该方法在0~5 mg/L范围内有良好的线性关系,相关系数r>0.995,在不同基质中,不同的添加水平下该方法的平均回收率范围在80.9%~101%,相对标准偏差为1.17%~12.60%;定量下限为0.030 mg/kg.结论此方法灵敏度高、准确、重现性好,适用于动物组织中阿布拉霉素残留量的检测.Objective To establish a method for the determination of apramycin in animal tissues by LC-MS/MS. Methods The residues of apramycin in the test samples were extracted with 5% trichloroacetic acid solution. After being cleaned up with SPE column, concentrated and reconstituted, the residues were detected by liquid chromatography-mass spectrometry under multiple reaction monitoring (MRM) mode via positive-ion mode. Results The method showed a good linearity over the range of 0~5 mg/L for apramycin with r>0.995. The average recoveries were 80.9%~101%at three or four spiked levels in animal tissues, and the relative standard devia-tions were 1.17%~12.6%. The limit of quantitation was 0.030 mg/kg. Conclusion This method is highly sensi-tive, accurate and reproducible, and it is suitable for the detection of apramycin in animal tissues.

关 键 词：阿布拉霉素 高压液相色谱-串联质谱 固相萃取 动物组织 

分 类 号：S859.84[农业科学—临床兽医学]