绞股蓝皂苷成分的分离与鉴定  

Isolation and characterization of gypenosides from Gynostemma pentaphyllum

作　　者：刘慧敏[1] 杨静[1] 陈道金[1] 朴香兰[1] 

机构地区：[1]中央民族大学中国少数民族传统医学研究院,北京100081

出　　处：《食品安全质量检测学报》2013年第1期283-288,共6页Journal of Food Safety and Quality

基　　金：国家自然科学基金项目(30973960;81011140347;81181240306;81274186)~~

摘　　要：目的提取、分离、鉴定绞股蓝皂苷主要成分。方法利用硅胶柱色谱及反相半制备柱色谱等分离手段,分离、纯化绞股蓝皂苷成分,并用核磁共振波谱(1H-NMR、13C-NMR)、液相色谱离子阱飞行时间质谱(LCMS-IT-TOF)等方法鉴定化合物结构,并对相关成分的抑癌作用进行了研究。结果绞股蓝总提物的主要成分经分离得到2种达玛烷类皂苷,分别为绞股蓝皂苷gypenoside XLVI和gypenoside LVI,而且它们具有一定的抑制癌细胞增殖作用。结论利用硅胶柱色谱和反相半制备色谱方法从绞股蓝总提物中分离得到主要的皂苷成分,为进一步研究其抑癌有效成分提供了参考。Objective To establish a method for the isolation and characterization of gypenosides from Gynostemma pentaphyllum. Methods Gypenosides were isolated with chromatography using silica gel and reversed semi-preparative HPLC and identified with 1H-nuclear magnetic resonance spectrum, 13C- nuclear magnetic resonance spectrum and liquid chromatography-ion trap time of flight tandem mass spectrometry. Results The two constituents isolated from Gynostemma pentaphyllum were identified as dammarane-type saponins, gypenoside XLVI and gypenoside LVI, respectively. Conclusion Ggypenosides can be isolated ef-fectively by silica gel chromatography and reversed semi-preparative HPLC.

关 键 词：绞股蓝 达玛烷类皂苷 GYPENOSIDE XLVI GYPENOSIDE LVI 

分 类 号：R284[医药卫生—中药学]