F_0F_1-ATPase生物传感器检测肠出血性大肠埃希菌O157:H7  

Detection of Escherichia coli O157:H7 by F_0F_1-ATPase immune biosensors

作　　者：魏玲[1] 李越[1] 李宝明[1] 伦永志[2] 张小莉[1] 亢子佳[1] 武会娟[1] 

机构地区：[1]北京市理化分析测试中心,北京市食品安全分析测试工程技术研究中心,北京100089 [2]大连大学医学院,大连116622

出　　处：《食品安全质量检测学报》2013年第2期433-438,共6页Journal of Food Safety and Quality

基　　金：北京市科学技术研究院萌芽计划项目(2011-45)~~

摘　　要：目的研究F0F1-ATPase纳米生物传感器在肠出血性大肠埃希菌O157:H7检测中的应用,开发高灵敏、特异性强的快速检测技术。方法利用免疫技术构建信号识别元件,根据F0F1-ATPase酶学机制建信号转化系统,建立免疫生物传感器,最后通过荧光扫描仪进行信号检测。本研究以食品中常见的大肠埃希菌(Escherichiacoli)和沙门菌(Salmonellatyphi)为干扰菌株进行了该方法的特异性研究。结果检测时间为4.5h,102～104cfu呈现良好的梯度性和线性,R2=0.9818。空白对照组和102cfu/孔均与104cfu/孔组存在极显著性差异,P值分均为P=0.00<0.01。结论 F0F1-ATPase纳米生物传感器对O157:H7检测快速、灵敏、特异性好,有良好的应用前景。@@@@Objective To develop a high sensitivity and specificity method for the detection of Es-cherichia coli O157:H7 by F0F1-ATPase immunobiosensors. Methods An immune biosensor based on the in-terference of load to the F0F1-ATPase rotation indicated by the fluorescence fluctuation was constructed to de-tect this pathogen. The concentration targets pathogen was measured according to the relative fluoresent value of F0F1-ATPase biosensor tagged by F1300 E.coli(ATCC14028) and Salmonella(CMCC44001) were tested as interferential bacteria to study the specificity of this method to O157: H7. Results The results demonstrated a good linearity (R2 = 0.9818) between antigen concentration (range from102 cfu to 104cfu) and the fluorescence intensity. The detection signals of the samples containing 102 cfu/well and 104 cfu/well O157: H7 were much stronger than the signal from control sample (P<0.01). Conclusion Owing to its much higher sensibility and simplicity than other currently applied methods, it provides a promising future for its application in food source pathogen detection.

关 键 词：O157:H7 免疫生物传感器 检测 

分 类 号：TS207.4[轻工技术与工程—食品科学] TP212.3[轻工技术与工程—食品科学与工程]