实时荧光RT-PCR检测冷冻草莓中诺如病毒  

Detection of norovirus in frozen strawberry by real-time fluorescent RT-PCR

作　　者：周阳[1,2] 栾军 蒋鲁岩[3] 唐泰山[3] 杨军[4] 张常印 张弛[4] 付瑞燕 祝长青[3] 

机构地区：[1]安徽农业大学茶与食品科技学院,合肥230000 [2]安徽省食品安全分析与检测省级实验室,合肥230000 [3]江苏出入境检验检疫局,南京210001 [4]南京市产品质量监督检验院,南京210028

出　　处：《食品安全质量检测学报》2013年第2期515-520,共6页Journal of Food Safety and Quality

基　　金：青奥会食品安全保障关键技术应用与示范项目(2011BAK21B05)~~

摘　　要：目的建立冷冻草莓中的GI、GII型诺如病毒实时荧光RT-PCR检测方法,并应用于实际样品的检测。方法对草莓样品进行前处理、病毒富集、病毒RNA的提取和纯化,然后采用实时荧光RT-PCR进行检测。结果核酸提取方法能够有效地去除抑制因子,同时对104份送检样品进行检测,结果均为阴性。结论所建立的核酸提取与实时荧光RT-PCR结合的检测体系适合于草莓样品中诺如病毒GI、GII型的检测。@@@@Objective To establish the real-time fluorescence RT-PCR detection method of norovirus GI and GII in frozen strawberry, and to apply to actual sample testing. Methods After pretreation of strawberry samples, concentrating of viruses, extracting and purifying of virus RNA, the samples were determinated by real-time fluorescence RT-PCR. Results The nucleic acid extraction method could effectively remove the in-hibitory factors. According to the test of 104 inspection samples, the results were all negative. Conclusion The established system of nucleic acid extraction and real-time fluorescence RT-PCR detection method is suit-able for the detection of norovirus GI and GII in frozen strawberry samples.

关 键 词：冷冻草莓 诺如病毒 实时荧光 RT-PCR 快速检测 

分 类 号：R155.5[医药卫生—营养与食品卫生学]