毛细管电泳法测定甘蔗中3-硝基丙酸  

Determination of 3-nitropropionic acid in sugarcane by capillary electrophoresis

作　　者：解娜[1,2] 丁晓静[1,3] 张晶[1] 赵珊[1] 王志[2] 

机构地区：[1]北京市疾病预防控制中心食物中毒诊断溯源技术北京市重点实验室,北京100013 [2]河北农业大学理学院,保定071001 [3]首都医科大学公共卫生学院,北京100069

出　　处：《食品安全质量检测学报》2013年第3期710-714,共5页Journal of Food Safety and Quality

摘　　要：目的建立甘蔗中3-硝基丙酸的毛细管电泳分析方法。方法粉碎后的甘蔗经样品提取液提取,离心除杂后,在228 nm波长处用毛细管电泳法检测。结果在优化的实验条件下,3-硝基丙酸的质量浓度在1.0～100.0 mg/L范围内与其对应的校正峰面积呈良好线性关系(r=0.9997)。方法检出限(S/N=3)为0.3 mg/L,定量限(S/N=10)为1.0 mg/L。在10.0、50.0 mg/L两个添加水平下,方法的回收率分别为95.2%和103.9%,相对标准偏差(n=5)分别为2.2%和1.3%。结论本方法简便、灵敏、准确,可用于甘蔗中3-硝基丙酸的测定。Objective To develop a method for the determination of 3-nitropropionic acid(3-NPA) in sugarcane by capillary electrophoresis.Methods After grinding,the sugarcane was extracted by sample buffer,centrifugated to remove impurity,analysed by capillary electrophoresis under the wavelength of 228 nm.Results Under the optimized conditions,a good linear relationship between values of corrected peak area and mass concentration of 3-NPA was obtained in the range of 1.0～100.0 mg/L with a correlation coefficient of 0.9997.The detection limit of the method was 0.3 mg/L(S/N=3) and the limit of quantification was 1.0 mg/L(S/N=10).The average recoveries at the spiked levels of 10.0 mg/L and 50.0 mg/L were 95.2% and 103.9% with relative standard derivations 2.2% and 1.3%,respectively.Conclusion This method is simple,sensitive and accurate,and it can be applied to the analysis of 3-NPA in sugarcane

关 键 词：3-硝基丙酸 甘蔗 毛细管电泳 

分 类 号：TS247[轻工技术与工程—制糖工程] O657.8[轻工技术与工程—食品科学与工程]