四氢大麻酚酸检测试纸条的研制  

Preparation of tetrahydrocannabinolic acid test strip

作　　者：黄书铭[1] 冀利峰[1,2] 郑曙剑 

机构地区：[1]合肥学院生物与环境工程系,合肥230601 [2]杭州隆基生物技术有限公司,杭州311121

出　　处：《食品安全质量检测学报》2013年第4期1228-1233,共6页Journal of Food Safety and Quality

摘　　要：目的利用胶体金免疫层析技术对因药物滥用致使尿液中产生的四氢大麻酚酸检测试纸条进行研制。方法采用柠檬酸三钠法还原氯金酸制备胶体金。结果确立了制备四氢大麻酚酸检测试纸条的最佳条件:标记10μg/mL的抗四氢大麻酚酸单克隆抗体,将金标抗体按喷量2.0μL/cm喷涂在已处理的聚酯膜上,最后将四氢大麻酚酸-牛血清白蛋白结合物和羊抗鼠按0.5、1.0mg/mL包被在硝酸纤维素膜(NC膜)上,经过37℃烘箱干燥16h,组装制备四氢大麻酚酸检测试纸条。结论这种方法能在3～5min内快速检测出尿液中残留的最小浓度为50ng/mL的四氢大麻酚酸。Objective To develop a tetrahydrocannabinol (THC) acid test strip by colloidal gold immu-nochromatographic technique. Methods Colloidal gold was prepared through reduction of chloroauric acid by trisodium citrate. Results The optimal conditions for preparing the THC acid test strip were as follows:10 μg/mL anti-THC acid monoclonal antibody was labeled, then the gold-labeled antibody was sprayed on a treated polyester film at a volume of 2.0 μL/cm, finally 0.5 mg/mL tetrahydrocannabinol acid-BSA combina-tion and 1.0 mg/mL goat anti-mouse antibody were used to coat the nitrocellulose (NC) membrane. After oven drying at 37 ℃ for 16 h, the tetrahydrocannabinol acid detection reagent was assembled. Conclusion The established method can rapidly detect residual THC acid at a minimum concentration of 50 ng/mL in urine in 3~5 min.

关 键 词：四氢大麻酚酸 胶体金法 尿液 免疫层析技术 

分 类 号：R446.6[医药卫生—诊断学]