一株类胡萝卜素高产菌的筛选与鉴定  

Isolation and Identification of a Bacterium Strain with High Production Yield of Carotenoid

作　　者：罗金亮[1] 周礼红[1] 陈平[1] 

机构地区：[1]贵州大学生命科学学院/真菌资源研究所,贵阳550025

出　　处：《湖北农业科学》2013年第10期2389-2392,共4页Hubei Agricultural Sciences

摘　　要：以自然环境中松树林土壤、下水道淤泥、饭店周围油腻土壤、农田土壤、落花、十字花科蔬菜、辣椒以及实验室空气为类胡萝卜素产生菌的初分离材料,采用酸-热破壁法用丙酮提取类胡萝卜素,根据单位体积发酵液类胡萝卜素产量确定了一株高产光合细菌(BSⅡ6)。进一步对其形态、生理生化特征、类胡萝卜素产量、分子生物学特征进行了初步研究,初步鉴定菌株BSⅡ6为无色杆菌属的一个种Achromobac-ter sp.,BSⅡ6所产色素主要成分为番茄红素,类胡萝卜素产量达到了7.46μg/mL。Pine forest soil,sewage sludge,oily soil around hotels,farmland soil,blossom dropping,cruciferous vegetables and pepper in nature environment,and laboratory air were used as the first isolated material for carotenoid producing strains.The carotenoid was extracted by acid-heat cell wall-breaking method with acetone.A high-yield photosynthetic bacterium strain(BSⅡ6) was isolated according to the carotenoid yield per unit volume of fermentation broth.Furthermore,the morphological,physiological and biochemical characteristics,carotenoid production yield and molecular characteristics of the strain was studied.The results showed that strain BSⅡ6 was preliminarily identified as Achromobacter sp.The main pigment component produced by BSⅡ6 was lycopene;with carotenoid yield up to 7.46 μg / mL.

关 键 词：类胡萝卜素 菌种 筛选 鉴定 发酵 无色杆菌属(Achromobacter) 

分 类 号：S[农业科学]