338例孕中期羊水细胞染色体核型结果分析  

Karyotype Analysis of Chromosome in Amniotic Fluid Cells of 338 Women at the Second Trimester of Pregnancy

作　　者：詹福寿[1] 陈耀平[1] 万艳 王贵杰[1] 霍正浩 魏军[1] 

机构地区：[1]宁夏医科大学总医院医学实验中心,银川750004 [2]解放军陆军第五医院,银川750004 [3]宁夏生殖与遗传重点实验室,银川750004

出　　处：《宁夏医科大学学报》2013年第7期770-772,共3页Journal of Ningxia Medical University

摘　　要：目的分析孕中期行产前诊断孕妇羊水细胞染色体核型,探讨羊水染色体检查对诊断胎儿染色体病的临床意义。方法对338例孕中期具有产前诊断指征的孕妇行羊膜腔穿刺术抽羊水进行细胞培养、G显带技术及染色体核型分析。结果羊水细胞培养成功率为100%。检出正常变异染色体36例(10.65%);检出异常核型13例(3.85%),其中数目异常9例,占异常核型的69.23%;结构异常4例,在异常核型中占30.77%。结论对具有产前诊断指征的孕妇进行羊水细胞染色体核型分析,能安全、有效地对胎儿染色体疾病进行产前诊断。Objective To analyze chromosome karyotype of amniotic fluid of pregnant women at the second trimester of pregnancy in prenatal diagnosis and to explore the clinical significance of karyotype analysis of amniotic fluid cells in prenatal diagnosis of chromosome abnormality disease.Methods 338 cases of pregnant women at the second trimester of pregnancy with the indications of prenatal diagnosis were implemented for amniocentesis,then amniotic fluid were cultured and performed the G-banding staining for karyotype analysis.Results The success rate of amniotic fluid cells culture was 100% in 338 cases of amniotic fluid specimen.36 cases of normal variation of chromosome were found(10.65%) and 13 cases of abnormal chromosome were found(3.85%) which included 9 of numerical abnormalities chromosome(69.23% in abnormal chromosome) and 4 of structural abnormalities chromosome(30.77% in abnormal chromosome) Conclusion To apply chromosomal karyotype analysis in amniotic fluid cells and prenatal diagnosis for the pregnant women with indications of prenatal diagnosis.can be safe and effective for fetal chromosome abnormalities in prenatal diagnosis.

关 键 词：羊水细胞 染色体异常 核型分析 产前诊断 

分 类 号：R714.55[医药卫生—妇产科学]