Embryoid body formation from embryonic and induced pluripotent stem cells:Benefits of bioreactors  

作　　者：Sasitorn Rungarunlert Mongkol Techakumphu Melinda K Pirity Andras Dinnyes 

机构地区：[1]Agricultural Biotechnology Center, H-2100 Gdll,Hungary [2]Andras Dinnyes,Molecular Animal Biotechnology Laboratory,Szent Istvan University,H-2100 Gdll,Hungary [3]Department of Obstetrics,Gynaecology and Reproduction,Faculty of Veterinary Science,Chulalongkorn University,Bangkok 10330,Thailand [4]BioTalentum Ltd.,Aulich Lajos u.26.H-2100,Gdll,Hungary

出　　处：《World Journal of Stem Cells》2009年第1期11-21,共11页世界干细胞杂志（英文版）（电子版）

基　　金：Supported by Grants from EU FP6("MEDRAT"-LSHG-CT-2005-518240;"CLONET",MRTN-CT-2006-035468),EU FP7("Partn ErS",PIAP-GA-2008-218205;"InduHeart",EU FP7-PEOPLE-IRG-2008-234390;"InduStem",PIAP-GA-2008-230675;"Plurisys",HEALTH-F4-2009-223485);NKFP_07_1-ES2HEART-HU,No.OM-00202-2007 ;CHE-TRF senior scholarship,No.RTA 5080010;supported by grant under the program Strategic Scholarships for Frontier Research Network for the Joint Ph.D.;Program Thai Doctoral degree from the Office of the Higher Education Commission,Thailand,No.CHE-PhD-SW-2005-100

摘　　要：Embryonic stem(ES)cells have the ability to differ-entiate into all germ layers,holding great promise not only for a model of early embryonic development but also for a robust cell source for cell-replacement therapies and for drug screening.Embryoid body (EB)formation from ES cells is a common method for producing different cell lineages for further applications. However,conventional techniques such as hanging drop or static suspension culture are either inherently incapable of large scale production or exhibit limited control over cell aggregation during EB formation and subsequent EB aggregation.For standardized mass EB production,a well defined scale-up platform is necessary.Recently,novel scenario methods of EB formation in hydrodynamic conditions created by bioreactor culture systems using stirred suspension systems(spinner flasks),rotating cell culture system and rotary orbital culture have allowed large-scale EB formation.Their use allows for continuous monitoring and control of the physical and chemical environment which is difficult to achieve by traditional methods.This review summarizes the current state of production of EBs derived from pluripotent cells in various culture systems.Furthermore,an overview of high quality EB formation strategies coupled with systems for in vitro differentiation into various cell types to be applied in cell replacement therapy is provided in this review. Recently,new insights in induced pluripotent stem(iPS) cell technology showed that differentiation and lineage commitment are not irreversible processes and this has opened new avenues in stem cell research.These cells are equivalent to ES cells in terms of both self-renewal and differentiation capacity.Hence,culture systems for expansion and differentiation of iPS cells can also apply methodologies developed with ES cells,although direct evidence of their use for iPS cells is still limited.Embryonic stem (ES) cells have the ability to differentiate into all germ layers, holding great promise not only for a model of early embryonic development but also for a robust cell source for cell-replacement therapies and for drug screening. Embryoid body (EB) formation from ES cells is a common method for producing different cell lineages for further applications. However, conventional techniques such as hanging drop or static suspension culture are either inherently incapable of large scale production or exhibit limited control over cell aggregation during EB formation and subsequent EB aggregation. For standardized mass EB production, a well defined scale-up platform is necessary. Recently, novel scenario methods of EB formation in hydrodynamic conditions created by bioreactor culture systems using stirred suspension systems (spinner flasks), rotating cell culture system and rotary orbital culture have allowed large-scale EB formation. Their use allows for continuous monitoring and control of the physical and chemical environment which is difficult to achieve by traditional methods. This review summarizes the current state of production of EBs derived from pluripotent cells in various culture systems. Furthermore, an overview of high quality EB formation strategies coupled with systems for in vitro differentiation into various cell types to be applied in cell replacement therapy is provided in this review. Recently, new insights in induced pluripotent stem (iPS) cell technology showed that differentiation and lineage commitment are not irreversible processes and this has opened new avenues in stem cell research. These cells are equivalent to ES cells in terms of both self-renewal and differentiation capacity. Hence, culture systems for expansion and differentiation of iPS cells can also apply methodologies developed with ES cells, although direct evidence of their use for iPS cells is still limited.

关 键 词：EMBRYOID body EMBRYONIC STEM CELLS Induced PLURIPOTENT STEM CELLS Bioreactors Different- iation 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]