Expression of PI3-K,PKB and GSK-3β in the skeletal muscle tissue of gestational diabetes mellitus  

作　　者：Tao Zhang Min Fang Zi-Mu Fu He-Chun Du Hua Yuan Gui-Yu Xia Jie Feng Gui-Yun Yin 

机构地区：[1]Shaoxing Women and Children's Hospital [2]Department of Gynaecology,Linyi People's Hospital

出　　处：《Asian Pacific Journal of Tropical Medicine》2014年第4期309-312,共4页亚太热带医药杂志（英文版）

基　　金：supported by Medical Fund of Zhejiang Province(No.2013KYA207);Shaoxing Science and Technology Bureau Program(No.2011A23013 and No.2013B70079)

摘　　要：Objective:To analyze the expression of phosphatidylinositol 3 kinase(PI3-K),protein kinase B(PKB)and glycogen synthase kinase 3 beta(GSK-3β)in skeletal muscle tissue of gestational diabetes mellitus(GDM).Methods:A total of 90 cases of pregnant women were divided into observation group and control group according to the occurrence of GDM with 45 cases in either,and the expression of PI3-K,PKB,GSK-3βmRNA expression in skeletal muscle tissue was compared between two groups.Results:The total PI3-K p85 protein was significantly higher in the observation group compared with the control group,the activity of PI3-K was lower than that of the latter;The total PKB,GSK-3βprotein in skeletal tissue had no significant difference between two groups,while the serine phosphorylation levels of PKB and GSK-3βwere significantly lower in observation group compared with the control group.Conclusions:The downregulation of PI3-K,PKB and GSK-3βin skeletal tissue of GDM caused by phosphorylation dysfunction of signaling molecules is the reason for insulin resistance and transporter function decline which lead to GDM.Objective:To analyze the expression of phosphatidylinositol 3 kinase(PI3-K),protein kinase B(PKB)and glycogen synthase kinase 3 beta(GSK-3β)in skeletal muscle tissue of gestational diabetes mellitus(GDM).Methods:A total of 90 cases of pregnant women were divided into observation group and control group according to the occurrence of GDM with 45 cases in either,and the expression of PI3-K,PKB,GSK-3βmRNA expression in skeletal muscle tissue was compared between two groups.Results:The total PI3-K p85 protein was significantly higher in the observation group compared with the control group,the activity of PI3-K was lower than that of the latter;The total PKB,GSK-3βprotein in skeletal tissue had no significant difference between two groups,while the serine phosphorylation levels of PKB and GSK-3βwere significantly lower in observation group compared with the control group.Conclusions:The downregulation of PI3-K,PKB and GSK-3βin skeletal tissue of GDM caused by phosphorylation dysfunction of signaling molecules is the reason for insulin resistance and transporter function decline which lead to GDM.

关 键 词：PHOSPHATIDYLINOSITOL 3 KINASE Protein KINASE B GLYCOGEN synthase KINASE 3β GESTATIONAL diabetes mellitus Skeletal musclet 

分 类 号：R714.2[医药卫生—妇产科学]