Expression and significance of p53 and mdm2 in patients with leukoplakia cancer  

作　　者：Juan-Juan Cui Xiao-Lan Han Wei-Min Wang 

机构地区：[1]Department of Stomatology,the First Affiliated Hospital of Anhui Medical University

出　　处：《Asian Pacific Journal of Tropical Medicine》2013年第10期831-834,共4页亚太热带医药杂志（英文版）

基　　金：supported by Education Department of Anhui(NoKJ2012Z148)

摘　　要：Objective:To study the relationship of the expressions of p53 and mdm2 in leukoplakia cancer.Methods:RT-PCR was used to detect the mRNA of p53,mdm2 in patients with leukoplakia cancer.The frequencies of p53,mdm2 in peripheral blood were detected by flow cytometric analysis.Results:The expression of p53mRNA in normal oral mucosa,simple oral leukoplakia,no-simple oral leukoplakia and leukoplakia cancer were 7.7%,27.3%,33.3%,56.8%,respectively.The frequencies of p53 in normal oral mucosa,simple oral leukoplakia,no-simple oral leukoplakia and leukoplakia cancer were(0.3±0.1)%,(l.6±0.9)%,(1.9±1.1)%,(3.4±1.8)%.The expression of mdm2 mRNA in normal oral mucosa,simple oral leukoplakia,no-simple oral leukoplakia and leukoplakia cancer were 0.0%,6.8%,11.1%,37.8%,respectively.The frequencies of mdm2 in normal oral mucosa,simple oral leukoplakia,no-simple oral leukoplakia and leukoplakia cancer were(0.1±0.1)%,(0.8±0.6)%,(1.2±0.8)%,(1.2±0.8)%.There was a positively correlation between p53 mRNA and mdm2 mRNA.Conclusions:The positive rate of p53 and mdm2 cells in the peripheral blood increases in patients with leukoplakia cancer tissue and has positive correlation with the severity of leukoplakia cancer.Objective:To study the relationship of the expressions of p53 and mdm2 in leukoplakia cancer.Methods:RT-PCR was used to detect the mRNA of p53,mdm2 in patients with leukoplakia cancer.The frequencies of p53,mdm2 in peripheral blood were detected by flow cytometric analysis.Results:The expression of p53mRNA in normal oral mucosa,simple oral leukoplakia,no-simple oral leukoplakia and leukoplakia cancer were 7.7%,27.3%,33.3%,56.8%,respectively.The frequencies of p53 in normal oral mucosa,simple oral leukoplakia,no-simple oral leukoplakia and leukoplakia cancer were(0.3±0.1)%,(l.6±0.9)%,(1.9±1.1)%,(3.4±1.8)%.The expression of mdm2 mRNA in normal oral mucosa,simple oral leukoplakia,no-simple oral leukoplakia and leukoplakia cancer were 0.0%,6.8%,11.1%,37.8%,respectively.The frequencies of mdm2 in normal oral mucosa,simple oral leukoplakia,no-simple oral leukoplakia and leukoplakia cancer were(0.1±0.1)%,(0.8±0.6)%,(1.2±0.8)%,(1.2±0.8)%.There was a positively correlation between p53 mRNA and mdm2 mRNA.Conclusions:The positive rate of p53 and mdm2 cells in the peripheral blood increases in patients with leukoplakia cancer tissue and has positive correlation with the severity of leukoplakia cancer.

关 键 词：ORAL LEUKOPLAKIA P53 MDM2 RT-PCR 

分 类 号：R739.8[医药卫生—肿瘤]