Isolation and identification of multidrug-resistant Staphylococcus haemolyticus from a laboratory-breeding mouse  

作　　者：Fengying Huang Qiuping Meng Guanghong Tan Yonghao Huang Hua Wang Wenli Mei Haofu Dai 

机构地区：[1]Hainan Provincial Key Laboratory of Tropical Medicine,Hainan Medical College [2]Agriculture School,Hainan University [3]Biological Technology Institute,Chinese Academy of Tropical Agricultural Sciences

出　　处：《Asian Pacific Journal of Tropical Medicine》2011年第6期421-425,共5页亚太热带医药杂志（英文版）

基　　金：partially funded by the National Natural Science Foundation of China(No.30960411);973 Program(No.2010CB534909)

摘　　要：Objective:To analysis and identify a bacterium strain isolated from laboratory breeding mouse far away from a hospital.Methods:Phenotype of the isolate was investigated by conventional microbiological methods,including Gram-staining,colony morphology,tests for haemolysis, catalase,coagulase,and antimicrobial susceptibility test.The meek and 16S rRNA genes were amplified by the polymerase chain reaction(PCR) and sequenced.The base sequence of the PCR product was compared with known 16S rRNA gene sequences in the CenBank database by phylogenetic analysis and multiple sequence alignment.Results:The isolate in this study was a gram positive,coagulase negative,and catalase positive coccus.The isolate was resistant to oxacillin,methicillin,penicillin,ampicillin,cefazolin,cipr of loxacin erythromycin,et al.PCR results indicated that the isolate was meek gene positive and its 16S rRNA was 1465 bp.Phylogenetic analysis of the resultant 16S rRNA indicated the isolate belonged to genus Saphylococcus,and multiple sequence alignment showed that the isolate was Saphylococcus haemolyticus with only one base difference from the corresponding 16S rRNA deposited in the CenBank.Conclusions: 16S rRNA gene sequencing is a suitable technique for non-specialist researchers.Laboratory animals are possible sources of lethal pathogens,and researchers must adapt protective measures when they manipulate animals.Objective: To analysis and identify a bacterium strain isolated from laboratory breeding mouse far away front a hospital. Methods: Phenotype of the isolate was investigated by conventional microbiological methods, including Gram-staining, colony morphology, tests for haemolysis, catalase, coagulase, and antimicrobial susceptibility test. The mecA and 16S rRNA genes were amplified by the polymerase chain reaction (PCR) and sequenced. The base sequence of the PCR product was compared with known 16S rRNA gene sequences in the GenBank database by phylogenetic analysis and multiple sequence alignment. Results: The isolate in this study was a gram positive, coagulase negative, and catalase positive coccus. The isolate was resistant to oxacillin, methicillin, penicillin, ampicillin, cefazolin, ciprofloxacin erythromycin, et al. PCR results indicated that the isolate was mecA gene positive and its 16S rRNA was 1 465 bp. Phylogenetic analysis of the resultant 16S rRNA indicated the isolate belonged to genus Saphylococcus, and multiple sequence alignment showed that the isolate was Saphylococcus haemolyticus with only one base difference from the corresponding 16S rRNA deposited in the GenBank. Conclusions: 16S rRNA gene sequencing is a suitable technique for non-specialist researchers. Laboratory animals are possible sources of lethal pathogens, and researchers must adapt protective measures when they manipulate animals.

关 键 词：16S RRNA Gene SEQUENCES analysis STAPHYLOCOCCUS haemolyticus MULTIDRUG resistant 

分 类 号：R378[医药卫生—病原生物学]