Inhibitory effect of two Indian medicinal plants on aldose reductase of rat lens in vitro  

作　　者：Rajesh Kumar Dinesh Kumar Patel Damiki Laloo Krishnamurthy Sairam Siva Hemalatha 

机构地区：[1]Deportment of Pharmaceutics,Institute of Technology.Banana Hindu University,Varanasi-221005,India

出　　处：《Asian Pacific Journal of Tropical Medicine》2011年第9期694-697,共4页亚太热带医药杂志（英文版）

摘　　要：Objective:To assesse the inhibitor) effect of alcoholic extract of two Indian medicinal plants namely Ceasalpinia digyna Rottler and.Alangium lamarckii Thwaits on aldose reductase（AR） of rat lens.Methods:Rats lens were enucleated through posterior approach and their homogenate was prepared and centrifuged to obtain a clear supernatant for the determination of AR activity and protein content.Results:The alcoholic extract of Ceasalpirda digyna and Alangium lamarckii had a potent inhibitory effect on the lens AR enzyme.The IC₅₀ values of alcoholic extract of the selected plants were calculated and were（46.29±11.17） and（106.00±5.11）μg/mL,respectively. Quercetin was used as a positive control and its IC₅₀ value was（2.95±1.53）μg/mL.Conclusions:Thus,it is concluded that alcoholic extracts of the selected plant exhibit significant inhibitory effects on AR in the rat lens in vitro.Objective:To assesse the inhibitor) effect of alcoholic extract of two Indian medicinal plants namely Ceasalpinia digyna Rottler and.Alangium lamarckii Thwaits on aldose reductase(AR) of rat lens.Methods:Rats lens were enucleated through posterior approach and their homogenate was prepared and centrifuged to obtain a clear supernatant for the determination of AR activity and protein content.Results:The alcoholic extract of Ceasalpirda digyna and Alangium lamarckii had a potent inhibitory effect on the lens AR enzyme.The IC_(50) values of alcoholic extract of the selected plants were calculated and were(46.29±11.17) and(106.00±5.11)μg/mL,respectively. Quercetin was used as a positive control and its IC_(50) value was(2.95±1.53)μg/mL.Conclusions:Thus,it is concluded that alcoholic extracts of the selected plant exhibit significant inhibitory effects on AR in the rat lens in vitro.

关 键 词：CAESALPINIA digyna Alangium lamarckii ALDOSE REDUCTASE DIABETIC CATARACT 

分 类 号：R285[医药卫生—中药学]