Evaluation of antigenotoxic effects of carotenoids from green algae Chlorococcum humicola using human lymphocytes  

作　　者：Bhagavathy S Sumathi P 

机构地区：[1]Bharathiar University,Coimbatore [2]Department of Biochemistry,Government Women's College,Krishnagiri

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2012年第2期109-117,共9页亚太热带生物医学杂志（英文版）

基　　金：supported by Bharathiar University.Tamilnadu,India

摘　　要：Objective:To identify the available phytochemicals and carotenoids in the selected green algae and evaluate the potential genotoxic/antigenotoxic effect using lymphocytes.Methods:Organic,solvent extracts of Chlorococcum humicola(C.humicola)were used for the phytochemical analysis.The available carotenoids were assessed by HPLC,and LC-MS analysis.The genotoxicity was induced by the benzo(a)pyrene in the lymphocyte culture,the genotoxic and antigenotoxic effects of algal carotenoids with and without genotoxic inducer were evaluated by chromosomal aberration(CA),sister chromatid exchange(SCE)and micronucleus assay(MN).Results:The results of the analysis showed that the algae were rich in carotenoids and fatty acids.In the total carotenoids lutein,β-carotene andα-carotene were found to be present in higher concentration.The frequency of CA and SCE increased by benzo(a)pyrene were significantly decreased by the carotenoids(P<0.05 for CA,P<0.001 for SCE).The MN frequencies of the cells were significantly decreased by the treatment with carotenoids when compared with the positive controls(P<0.05).Conclusions:The findings of the present study demonstrate that,the green algae C.humicola is a rich source of bioactive compounds especially carotenoids which effectively fight against environmental genotoxic agents,the carotenoids itself is not a genotoxic substance and should be further considered for its beneficial effects.Objective:To identify the available phytochemicals and carotenoids in the selected green algae and evaluate the potential genotoxic/antigenotoxic effect using lymphocytes.Methods:Organic solvenl extracts of Chlorococcum humicola(C.humicola) were used for the phytochemical analysis.The available carotenoids were assessed by HPLC,and LC-MS analysis.The genotoxicity was induced by the benzo(a)pyrene in the lymphocyte culture,the genotoxic and anligenoloxic effects of algal carotenoids with and without genotoxic inducer were evaluated In chromosomal aberration(CA),sister chromatid exchange(SCE) and micronucleus assay(MN).Results:The results of the analysis showed that the algae were rich in carotenoids and fatty acids.In the total carotenoids lutein.β-carotene and α-carotene were found to be present in higher concentration.The frequency of CA and SCE increased by benzo(a)pyrene were significantly decreased by the carotenoids(P<0.05 for CA,P<0.001 for SCE).The MN frequencies ol the cells were significantly decreased by the treatment with carotenoids when compared with the positive controls(P<0.05).Conclusions:The findings of the present study demonstrate that,the green algae C.humicola is a rich source of bioactive compounds especially carotenoids which effectively fight against environmental genotoxic agents,the carotenoids itself is not a genotoxic substance and should be further considered for its beneficial effects.

关 键 词：Chlorococcum humicola Benzo(a)pyrene GENOTOXICITY ANTIGENOTOXICITY CHROMOSOMAL aberration SISTER chromatid exchange MICRONUCLEUS assay Carotenoids Green algae 

分 类 号：R114[医药卫生—卫生毒理学]