乳腺癌中17号染色体倍体性分析  

Analysis of Chromosome 17 Polysomy in Invasive Breast Cancer

作　　者：陈顺平[1] 余英豪[2] 吴文乔[1] 周宗楷 谢飞来[2] 沈洪武[1] 王烈[2] 

机构地区：[1]福建医科大学附属漳州市医院,363000 [2]南军军区福州总医院,350025

出　　处：《实用癌症杂志》2014年第2期126-129,134,共4页The Practical Journal of Cancer

基　　金：卫生部科研资助项目(WKJ2007-3-001)

摘　　要：目的探讨乳腺癌中17号染色体倍体性及其与临床病理参数的关系。方法采用荧光原位杂交(FISH)及免疫组化(IHC),检测300例浸润性乳腺癌中人类表皮生长因子受体2(Her-2)表达和17号染色体倍体情况及ER、PR蛋白表达情况。结果 300例浸润性乳腺癌中,Her-2扩增者中17号染色体多体占55.44%,Her-2无扩增中共占25.13%(P<0.01);Her-2蛋白表达++/+++及Her-2蛋白表达-/+中17号染色体多体分别占59.12%、15.34%(P<0.01),17号染色体多体与ER、PR表达相关,与淋巴结转移及TNM分期无相关性。结论 17号染色体多体是乳腺癌常见遗传学改变,其多体性与乳腺癌病情发展无关。Objective To explore the chromosome 17 polysomy in breast cancer and its relationship with clinicopatho-logical parameters .Methods Fluorescence in situ hybridization ( FISH) and immunohistochemistry ( IHC) method was used to detect the amplification and expression of Human epidermal growth factor receptor 2 (Her-2),chromosome 17 polysomy and the expression of ER,PR in 300 cases of invasive breast cancer .Results Chromosome 17 polysomy was 55.44%in Her-2 amplifica-tion cases,and it was 25.13%in without Her-2 amplification cases(P<0.01).The rate of Chromosome 17 polysomy in Her-2 protein express ++/+++cases was 59.12%and in Her-2 protein express-/+cases was 15.34%(P<0.01).Chromo-some 17 polysomy was related to ER ,PR protein,but not significantly related to age ,lymph node metastasis and TNM staging . Conclusion Chromosome 17 polysomy is a common genetic change in breast cancer ,it is not related to Breast cancer progres-sion.

关 键 词：乳腺肿瘤 人类表皮生长因子受体2 17号染色体 原位杂交 荧光 免疫组化 

分 类 号：R737.9[医药卫生—肿瘤]