THE COCHlEAR RIBBON SYNAPTIC RESPONSE TO AMINOGIYCOSIDE OTOTOXICITY IN C57BL/6J MICE  

作　　者：LIU Ke ZHAO Ning SHI Chuang WU Nan LIU Huizhan ZHANG Yue YANG Weiyan YANG Shiming 

机构地区：[1]The Institute of Otolaryngology, PLA General Hospital [2]The Department of Otolaryngology, The First Affiliated Hospital of China Medical University [3]China Medical University

出　　处：《Journal of Otology》2014年第1期16-20,共5页中华耳科学杂志（英文版）

基　　金：supported by grants from the National Basic Research Program of China(973Program);Beijing Natural Science Foundation(5122040);supported by grants from the China Postdoctoral Science Foundation(201003779;20100470103);the National Natural Science Foundation of China(NSFC)(31040038)

摘　　要：Objective To investigate the early change of cochlear ribbon synapses on inner hair cells in response to aminoglycoside ototoxicity. Methods C57BL/6J mice received intraperitoneal injection of gentamicin (100 mg/kg/day), and the apical coil organ of Corti was examined on the 4th, 7th and 10th day (n=10). Litter-mates without gentamicin treatment served as controls (n=10). RIBEYE on the presynaptic membrane and AMPA receptors on the postsynaptic membrane were labeled with CtBP2 or GluR2/3 respectively. Three di-mension reconstruction was conducted using the 3DS MAX 8.0 software. Results There were no disruptions of outer or inner hair cells in all groups. However, the number of ribbon synapses on cochlear inner hair cells increased significantly within 7 days after gentamicin exposure (P<0.01), followed by a significant de-crease after 7 days.Conclusion During the early stage of aminoglycoside ototoxicity, increased population of cochlear ribbon synapses may indicate a significant down-regulation of synaptic function.Objective To investigate the early change of cochlear ribbon synapses on inner hair cells in response to aminoglycoside ototoxicity. Methods C57BL/6J mice received intraperitoneal injection of gentamicin (100 mg/kg/day), and the apical coil organ of Corti was examined on the 4th, 7th and 10th day (n=10). Litter-mates without gentamicin treatment served as controls (n=10). RIBEYE on the presynaptic membrane and AMPA receptors on the postsynaptic membrane were labeled with CtBP2 or GluR2/3 respectively. Three di-mension reconstruction was conducted using the 3DS MAX 8.0 software. Results There were no disruptions of outer or inner hair cells in all groups. However, the number of ribbon synapses on cochlear inner hair cells increased significantly within 7 days after gentamicin exposure (P<0.01), followed by a significant de-crease after 7 days.Conclusion During the early stage of aminoglycoside ototoxicity, increased population of cochlear ribbon synapses may indicate a significant down-regulation of synaptic function.

关 键 词：GENTAMICIN COCHLEA Cochlear ribbon synapse Confocal microscope 

分 类 号：R764.45[医药卫生—耳鼻咽喉科]