Advanced oxidation protein products decrease expression of nephrin and podocin in podocytes via ROS-dependent activation of p38 MAPK  

作　　者：YANG Li,LIANG Min,ZHOU QiuGen,XIE Di,LOU AiJu,ZHANG Xun & HOU FanFan Key Laboratory for Organ Failure Research,Division of Nephrology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China. 

出　　处：《Science China(Life Sciences)》2010年第1期68-77,共10页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant Nos 30830056 and 30830056);the National Basic Research Program of China (Grant No 2006CB503904) to Dr HOU FanFan;the National Natural Science Foundation of China (Grant No 30971382);the Natural Science Foundation of Guangdong Province (Grant No 06024402) to Dr LIANG Min

摘　　要：Accumulation of plasma advanced oxidation protein products(AOPPs) promotes progression of proteinuria and glomerulo-sclerosis.To investigate the molecular basis of AOPPs-induced proteinuria,normal Sprague-Dawley rats were treated with AOPPs-modified rat serum albumin.The expression of glomerular podocyte slit diaphragm(PSD)-associated proteins,nephrin and podocin,was significantly decreased coincident with the onset of albuminuria in rats treated with AOPPs.Chronic inhibi-tion of NADPH oxidase by apocynin prevented down-regulation of nephrin and podocin and decreased albuminuria in AOPPs-challenged rats.This suggested that accumulation of AOPPs promotes proteinuria,possibly via down-regulating the expression of PSD-associated proteins.Accumulation of plasma advanced oxidation protein products(AOPPs) promotes progression of proteinuria and glomerulo-sclerosis.To investigate the molecular basis of AOPPs-induced proteinuria,normal Sprague-Dawley rats were treated with AOPPs-modified rat serum albumin.The expression of glomerular podocyte slit diaphragm(PSD)-associated proteins,nephrin and podocin,was significantly decreased coincident with the onset of albuminuria in rats treated with AOPPs.Chronic inhibi-tion of NADPH oxidase by apocynin prevented down-regulation of nephrin and podocin and decreased albuminuria in AOPPs-challenged rats.This suggested that accumulation of AOPPs promotes proteinuria,possibly via down-regulating the expression of PSD-associated proteins.

关 键 词：advanced oxidation protein products PODOCYTE NEPHRIN PODOCIN NADPH OXIDASE 

分 类 号：Q51[生物学—生物化学]