Chloride channel-dependent copper acquisition of laccase in the basidiomycetous fungus Cryptococcus neoformans  

作　　者：ZHU ChangWei1,JIANG Nan1,XIAO DongGuang2,PAN Jiao1 & ZHU XuDong1 1 State Key Program of Microbiology and Department of Microbiology,College of Life Sciences,Nankai University,Tianjin 300071,China 2 Tianjin Industrial Microbiology Key Lab,College of Bioengineering,Tianjin University of Science & Technology,Tianjin 300457,China 

出　　处：《Science China(Life Sciences)》2010年第1期125-130,共6页中国科学（生命科学英文版）

基　　金：supported by the Natural Nature Science Foundation of China (Grant No 30770043);the National Basic Research Program of China (Grant No 2007CB707801);the Tianjin Science and Technology Development Plan (Grant No 06YFGSH00500)

摘　　要：The CLC chloride channel gene CLC-A of the pathogen yeast Cryptococcus neoformans was previously reported to be critical for multicopper laccase activity and growth at an elevated pH.This study reports that copper homeostasis was impaired in the clc-a mutant.This was demonstrated by the substantial decrease of the intracellular quantity of copper under copper-limited growth as determined by flame atomic absorption spectrometry.CLC-A is a critical factor in copper homeostasis which is required for copper acquisition of laccase in C.neoformans.The CLC chloride channel gene CLC-A of the pathogen yeast Cryptococcus neoformans was previously reported to be critical for multicopper laccase activity and growth at an elevated pH.This study reports that copper homeostasis was impaired in the clc-a mutant.This was demonstrated by the substantial decrease of the intracellular quantity of copper under copper-limited growth as determined by flame atomic absorption spectrometry.CLC-A is a critical factor in copper homeostasis which is required for copper acquisition of laccase in C.neoformans.

关 键 词：CHLORIDE channel LACCASE COPPER HOMEOSTASIS CRYPTOCOCCUS neoformans 

分 类 号：Q933[生物学—微生物学]