Short exposure to paclitaxel induces multipolar spindle formation and aneuploidy through promotion of acentrosomal pole assembly  

作　　者：BIAN MingLei1, FU JingYan1, YAN Yan1, CHEN Qiang1, YANG Chao2, SHI QingHua2,JIANG Qing1 & ZHANG ChuanMao1 1MOE Key Laboratory of Cell Proliferation and Differentiation and the State Key Laboratory of Bio-membrane and Membrane Bio-engineering, College of Life Sciences, Peking University, Beijing 100871, China 2Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230026, China 

出　　处：《Science China(Life Sciences)》2010年第11期1322-1329,共8页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant Nos.31071188,31030044,30721064,30900726 and 90913021);the National Key Basic Research and Development Program of China (Grant Nos.2010CB833705,2006CB910101 and 2007CB914502)

摘　　要：Paclitaxel is a widely used microtubule drug and cancer medicine. Here we report that by short exposure to paclitaxel at a low dose, multipolar spindles were induced in mitotic cells without centrosome amplification. Both TPX2 depletion and Aurora-A overexpression antagonized the multipolarity. Live cell imaging showed that some paclitaxel-treated cells accomplished multipolar cell division and a portion of the daughter cells went on to the next round of mitosis. The surviving cells grew into clones with varied genome content. The results indicated that an aneuploidy population could be induced by short exposure to paclitaxel at a low dose, implicating potential side effects of paclitaxel.Paclitaxel is a widely used microtubule drug and cancer medicine. Here we report that by short exposure to paclitaxel at a low dose, multipolar spindles were induced in mitotic cells without centrosome amplification. Both TPX2 depletion and Aurora-A overexpression antagonized the multipolarity. Live cell imaging showed that some paclitaxel-treated cells accomplished multipolar cell division and a portion of the daughter cells went on to the next round of mitosis. The surviving cells grew into clones with varied genome content. The results indicated that an aneuploidy population could be induced by short exposure to paclitaxel at a low dose, implicating potential side effects of paclitaxel.

关 键 词：PACLITAXEL TPX2 AURORA-A multipolar cell DIVISION ANEUPLOIDY 

分 类 号：R285.5[医药卫生—中药学]