What we have learned from transcript profile analyses of male and female gametes in flowering plants  

作　　者：XIN HaiPing & SUN MengXiang Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Science, Wuhan University, Wuhan 430072, China 

出　　处：《Science China(Life Sciences)》2010年第8期927-933,共7页中国科学（生命科学英文版）

基　　金：supported by the National Basic Research Program of China (Grant No. 2007CB108700);the National Natural Science Foundation of China (Grant Nos. 30821064 and 30771131)

摘　　要：Double fertilization is one of the predominant features of sexual reproduction in flowering plants but, because of the physical inaccessibility of gametes, the essential molecular mechanisms in these processes are largely unknown. Based on the techniques for isolating highly purified gametes from several species and well-developed methods for manipulating RNA from limited quantities of gametes, genome-wide investigations of gamete transcription profiles were recently conducted in flowering plants. In this review, we survey the accumulated knowledge on gamete collection and purification, cDNA library construction, and transcript profile analysis to assess our understanding of the molecular mechanisms of gamete specialization and fertilization.Double fertilization is one of the predominant features of sexual reproduction in flowering plants but, because of the physical inaccessibility of gametes, the essential molecular mechanisms in these processes are largely unknown. Based on the techniques for isolating highly purified gametes from several species and well-developed methods for manipulating RNA from limited quantities of gametes, genome-wide investigations of gamete transcription profiles were recently conducted in flowering plants. In this review, we survey the accumulated knowledge on gamete collection and purification, cDNA library construction, and transcript profile analysis to assess our understanding of the molecular mechanisms of gamete specialization and fertilization.

关 键 词：SPERM CELL egg CELL fertilization cDNA library GAMETOGENESIS TRANSCRIPT PROFILE 

分 类 号：Q945[生物学—植物学]