Dynamics of morphological changes for mitochondrial fission and fusion  

作　　者：WANG ShiQi 1,2,4 , FU ChangLiang 1,2 , ZHANG Yan 1,2 , CHEN Quan 3 & LONG Mian 1,2 1 Key Laboratory of Microgravity (National Microgravity Laboratory), Beijing 100190, China 2 Center for Biomechanics and Bioengineering, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100190, China 3 State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China 4 Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences, Beijing 100044, China 

出　　处：《Science China(Physics,Mechanics & Astronomy)》2010年第4期680-689,共10页中国科学：物理学、力学、天文学（英文版）

基　　金：supported by theNational Natural Science Foundation of China (Grant Nos. 30730032 and10332060);the National Key Basic Research Foundation of China (GrantNo. 2006CB910303);the National High Technology Research and Development Program of China (Grant No. 2007AA02Z306);Innovation Program of the Chinese Academy of Sciences (Grant No.KJCX2-YW-L08)

摘　　要：Mitochondria experience continuous fusion and fission in a living cell, but their dynamics remains poorly quantified. Here a theoretical model was developed, upon a simplified population balance equation (PBE), to predict the morphological changes induced by mitochondrial fission and fusion. Assuming that both fission and fusion events are statistically independent, the survival probability of mitochondria staying in the fission or fusion state was formulated as an exponentially-decayed function with time, which depended on the time-dependent distribution of the mitochondrial volume and the fission and fusion rates. Parametric analysis was done for two typical volume distributions. One was Gamma distribution and the other was Gaussian distribution, derived from the measurements of volume distribution for individual mitochondria in a living cell and purified mitochondria in vitro. The predictions indicated that the survival probability strongly depended on morphological changes of individual mitochondria and was inversely correlated to the fission and fusion rates. This work provided a new insight into quantifying the mitochondrial dynamics via monitoring the evolution of the mitochondrial volume.Mitochondria experience continuous fusion and fission in a living cell, but their dynamics remains poorly quantified. Here a theoretical model was developed, upon a simplified population balance equation (PBE), to predict the morphological changes induced by mitochondrial fission and fusion. Assuming that both fission and fusion events are statistically independent, the survival probability of mitochondria staying in the fission or fusion state was formulated as an exponentially-decayed function with time, which depended on the time-dependent distribution of the mitochondrial volume and the fission and fusion rates. Parametric analysis was done for two typical volume distributions. One was Gamma distribution and the other was Gaussian distribution, derived from the measurements of volume distribution for individual mitochondria in a living cell and purified mitochondria in vitro. The predictions indicated that the survival probability strongly depended on morphological changes of individual mitochondria and was inversely correlated to the fission and fusion rates. This work provided a new insight into quantifying the mitochondrial dynamics via monitoring the evolution of the mitochondrial volume.

关 键 词：MITOCHONDRIA MORPHOLOGY FISSION FUSION DYNAMICS MODELING 

分 类 号：Q244[生物学—细胞生物学]