Effect of Insulin on the Differential Expression of VLDL Receptor Isoforms of SGC7901 Cell and Its Biological Implication  

作　　者：蔡哲钧 李飞 彭传梅 李和 宗义强 刘志国 屈伸 

机构地区：[1]Department of Biochemistry and Molecular Biology,Tongji Medical College,Huazhong University of Science and Technology [2]School of Biology and Pharmaceutical Engineering,Wuhan Polytechnic University

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2010年第5期551-555,共5页华中科技大学学报（医学英德文版）

基　　金：supported by grants from National Natural Sciences Foundation of China (No.39970307);Hubei Provincial Natural Sciences Foundation of China (No.2005ABA092)

摘　　要：This study examined the effect of insulin on the expression of very low density lipoprotein receptor (VLDLR) subtypes of SGC7901 cells and discussed its biological implication.In vitro, moderately or poorly-differentiated human gastric adenocarcinoma cell line SGC7901 was incubated with insulin for different lengths of time, and then the expression of protein and RNA level in VLDLR subtypes were detected by Western blotting and real-time PCR, respectively.The results showed that, at certain time interval, insulin could down-regulate expression of type Ⅰ VLDLR and up-regulate the expression of type Ⅱ VLDLR in SGC7901 cells, at both protein and RNA level.We are led to conclude that insulin serves as a regulator in maintaining the balance between glucose and lipid metabolism in vivo, possibly through its effect on the differential expression of VLDLR subtypes.This study examined the effect of insulin on the expression of very low density lipoprotein receptor (VLDLR) subtypes of SGC7901 cells and discussed its biological implication.In vitro, moderately or poorly-differentiated human gastric adenocarcinoma cell line SGC7901 was incubated with insulin for different lengths of time, and then the expression of protein and RNA level in VLDLR subtypes were detected by Western blotting and real-time PCR, respectively.The results showed that, at certain time interval, insulin could down-regulate expression of type Ⅰ VLDLR and up-regulate the expression of type Ⅱ VLDLR in SGC7901 cells, at both protein and RNA level.We are led to conclude that insulin serves as a regulator in maintaining the balance between glucose and lipid metabolism in vivo, possibly through its effect on the differential expression of VLDLR subtypes.

关 键 词：INSULIN very low density lipoprotein receptor cell proliferation metabolic regulation 

分 类 号：R735.2[医药卫生—肿瘤]