鼠尾胶原建立胶滴肿瘤药敏检测方法的初步研究  

Preliminary studies on establishing the method of collagen-gel droplet embedded culture drug sensitivity test with rat tail collagen

作　　者：郭宁[1] 巩平[2] 高洪波[1] 孙洁[2] 王振华[3] 郑秋生[3] 

机构地区：[1]石河子大学医学院,新疆石河子832000 [2]石河子大学医学院第一附属医院肿瘤内科,新疆石河子832000 [3]石河子大学药学院,新疆石河子832000

出　　处：《现代生物医学进展》2009年第24期4639-4643,共5页Progress in Modern Biomedicine

摘　　要：目的:用鼠尾胶原建立胶滴肿瘤药敏检测方法(CD-DST),探讨此方法在体外药物敏感性检测中的可行性。方法:建立Hela细胞在鼠尾胶原中培养的三维立体模型;在此基础上进行药物敏感性检测,用MTT、CD-DST两种药敏检测方法检测Hela细胞对4种化疗药物的敏感性。结果:Hela细胞在鼠尾胶原凝胶滴中生长良好,呈克隆样增殖、放射状生长;两种药敏检测方法有较好的一致性。结论:用鼠尾胶原建立的CD-DST技术是可行的,在体外药敏检测中具有一定的临床应用价值。0bjective:To establish the method of collagen-gel droplet embedded culture drug sensitivity test with I rat tail collagen,and explore the clinical application of this method in vitro chemosensitivities test of tumors.Methods:To establish three diamensions formwork of rat tail collagen,then drug sensitivity of Hela cells to 4 chemotherapeutics is detected by MTT and CD-DST. Results:Hela cells grow well in collagen-gel droplet, clonal proliferation, radiated growth; there is a good concordance with 2 methods. Conclusions: CD-DST with rat tail collagen is feasible , and there is a clinical application value in vitro chemosensitivities test.

关 键 词：肿瘤化疗 鼠尾胶原 胶滴肿瘤药敏检测 

分 类 号：R730.5[医药卫生—肿瘤]