藻红蛋白诱导人乳腺癌MCF-7细胞衰老研究  

Study on the senescence induced by phycoerythrin in human breast cancer MCF-7

作　　者：季宇彬[1,2] 刘日嘉[1,2] 高世勇[1,2] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心药物研究所博士后科研工作站,黑龙江哈尔滨150076 [2]国家教育部抗肿瘤天然药物工程研究中心,黑龙江哈尔滨150076

出　　处：《药物评价研究》2009年第1期13-18,共6页Drug Evaluation Research

基　　金：国家自然科学基金项目(300672595);教育部科学技术研究重点项目(207030);教育部博士点基金项目(20070240081);黑龙江省自然科学基金重大项目(ZJY0504)

摘　　要：目的:研究藻红蛋白对人乳腺癌MCF-7细胞的诱导衰老作用。方法:采用MTT法检测藻红蛋白对MCF-7细胞的生长抑制作用,采用倒置显微镜观察形态学变化,酸性β-半乳糖苷酶染色法和衰老相关异染色质聚集检测藻红蛋白诱导人乳腺癌MCF-7细胞衰老作用。结果:MTT法结果显示,藻红蛋白对体外培养的人乳腺癌细胞MCF-7具有明显的生长抑制作用,IC50为134.87μg/mL。结果发现藻红蛋白作用72h后,细胞呈现变大变扁平,细胞内形成空泡、颗粒增多等明显衰老形态。在倒置显微镜下观察,酸性β-半乳糖苷酶染色呈阳性;在荧光显微镜下观察,DAPI染色出现衰老相关异染色质聚集现象。结论:藻红蛋白可通过诱导MCF-7细胞衰老达到抗肿瘤作用。Objective:To investigate the effects of phycoerythrin(PE) on the induction of cell senescence in human breast cancer MCF-7 cells.Methods:The inhibitory rate of MCF-7 cells growth was measured by MTT assay and morphological changes of MCF-7 cells was observed through the inverted microscope.Acidic β-galactosidase dying(SA-β-Gal dying) and senescence-associated heterochromatin aggregation were used to detect the cell senescence caused by PE in MCF-7 cells.Results:The results of MTT assay indicates that PE could inhibit the growth of human breast cancer MCF-7 cells significantly and the IC50 was 134.87μg/mL.After 72 h,MCF-7 cells which were treated by PE had become bigger and flatter meanwhile the appearance of vacuolization and particles which are the specific patterns of the cell senescence were also observed.The staining of acidic β-galactosidase was positive,which observed by inverted microscope,and, the emergence of senescence-associated heterochromatin aggregation(SAHF) in staining of DAPI was found under the fluorescence microscope.Conclusion:The results demonstrate that PE has the antitumor effect by inducing the cell senescence of MCF-7 cells.

关 键 词：藻红蛋白 细胞衰老 酸性β-半乳糖苷酶染色 衰老相关异染色质聚集 

分 类 号：R737.9[医药卫生—肿瘤]