Development of specific chromosomal DNA pool for rice field eel and their application to gene mapping  

作　　者：Ji, FY Yu, QX Liu, JD 

机构地区：[1]Wuhan Univ, Ctr Dev Biol, Wuhan 430072, Peoples R China

出　　处：《Chinese Science Bulletin》2002年第6期485-489,共5页

基　　金：This work was supported by the National Natural Science Foundation of China (Grant No. 39970406);the Research Fund for the Doctoral Program of Higher Education of China (Grant No. 98048628).

摘　　要：The chromosomes 1, 3, 5, 6, 7,10 and 12 of rice field eel (Monopterus albus Zuiew) have been microdissected successfully from meiosis I diakinesis spreads by using glass microneedlc under an inverted microscope. And the DOP-PCR products of the single chromosome dotted on the nylon membrane as 'specific chromosomal DNA pool', have been hybridized with 6 probes to map these genes. The mapping results show that Zfa has been mapped to chromosome 1, rDNA to chromosomes 3 and 7, both Gh and Pdegγto chromosome 10, Hsl to chromosome 5 and Hox genes have been detected on chromosomes 1, 3, 6 and 10 meantime. It has initiatively been suggested that chromosome 10 of rice field eel might possess the commom conserved synteny to that on chromosome 17 of human, chromosome 11 of mouse, chromosome 12 of pig and chromosome 19 of bovine. And so chromosome 3 of rice field eel might also contain the commom conserved synteny to that on chromosome 2 of zebraf-ish. Our study is an attempt to establish a new and feasibleThe chromosomes 1, 3, 5, 6, 7, 10 and 12 of rice field eel (Monopterus albus Zuiew) have been microdissected successfully from meiosis I diakinesis spreads by using glass microneedlc under an inverted microscope. And the DOP-PCR products of the single chromosome dotted on the nylon membrane as 'specific chromosomal DNA pool', have been hybridized with 6 probes to map these genes. The mapping results show that Zfa has been mapped to chromosome 1, rDNA to chromosomes 3 and 7, both Gh and Pdegr to chromosome 10, Hsl to chromosome 5 and Hox genes have been detected on chromosomes 1, 3, 6 and 10 meantime. It has initiatively been suggested that chromosome 10 of rice field eel might possess the commom conserved synteny to that on chromosome 17 of human, chromosome 11 of mouse, chromosome 12 of pig and chromosome 19 of bovine. And so chromosome 3 of rice field eel might also contain the commom conserved synteny to that on chromosome 2 of zebraf-ish. Our study is an attempt to establish a new and feasible method to advance the study of gene mapping and chromosome evolution in fish, and also to provide a new idea to distinguish each chromosome on the base of molecular markers for fish.

关 键 词：rice field EEL MICRODISSECTION DOP-PCR SPECIFIC chro-mosomal DNA POOL gene mapping conserved synteny. 

分 类 号：Q943[生物学—植物学]