Isolation and purification of BV Ⅰ-2H from bee venom and analysis of its biological action  

Isolation and purification of BV I-2H from bee venom and analysis of its biological action

作　　者：ZHU Wei WANG Benxiang ZHU Xun 

机构地区：[1]Department of Immunology,Jilin University,Changchun 130021,China [2]Research Center of New Drug,Changchun College of Traditional Chinese Medicine,Changchun 130021,China

出　　处：《Chinese Science Bulletin》2002年第11期910-914,共5页

摘　　要：The medical use of bee venom for rheumatoid arthritis ( RA ) has a very long tradition. In this study, isolation and purification of polypeptides from bee venom were carried out on sephadex chromatography, heparin sepharose CL6B chromatography and HPLC. Several fractions were extracted, and their effects on activation of splenocyte and THP-1 cell were studied. The inhibitory fraction was selected for further studies. Finally, BV I-2H that the HPLC elution profiles was a single peak was isolated by C8 column. ESI-MS detection results showed that BY I-2H was a fraction of bee venom, and the molecular weight of the major component was 644.8. BY I -2H could inhibit ConA-induced splenocyte proliferation, IL-1 production and interfere with splenocyte cycle in mice. Moreover, BV I-2H could inhibit PMA-induced TNFa production in THP-1 cells, which was due to its inhibitory effects on TNFa mRNA expression and protein phosphorylation of IκBα. Our studies indicated that BY I -2H was one of the anti-inflammatoryThe medical use of bee venom for rheumatoid arthritis ( RA ) has a very long tradition. In this study, isolation and purification of polypeptides from bee venom were carried out on sephadex chromatography, heparin sepharose CL-6B chromatography and HPLC. Several fractions were extracted, and their effects on activation of splenocyte and THP-1 cell were studied. The inhibitory fraction was selected for further studies. Finally, BY I-2H that the HPLC elution profiles was a single peak was isolated by C8 column. ESI-MS detection results showed that BY I-2H was a fraction of bee venom, and the molecular weight of the major component was 644.8. BY I -2H could inhibit ConA-induced splenocyte proliferation, IL-1 production and interfere with splenocyte cycle in mice. Moreover, BY I-2H could inhibit PMA-induced TNFα production in THP-1 cells, which was due to its inhibitory effects on TNFα mRNA expression and protein phosphorylation of IkBα. Our studies indicated that BY I -2H was one of the anti-inflammatory components of bee venom.

关 键 词：POLYPEPTIDES of BEE VENOM SPLENOCYTE proliferation cytokines cell cycle IκBα. 

分 类 号：R259.9[医药卫生—中西医结合]