Investigation of protein-styrene oxide adducts as a molecular biomarker of human exposed to styrene  

作　　者：Liu, S.-F.  Fang, Q.-M.  Jin, Z.-L.  Rappaport, M.S. 

机构地区：[1] Res. Cntr. Eco-Environmental Sci., Chinese Academy of Sciences, Beijing 100085, China

出　　处：《Journal of Environmental Sciences》2001年第4期391-397,共7页环境科学学报（英文版）

基　　金：TheNationalNaturalScienceFoundationofChina (No .5 98730 2 8)

摘　　要：Hemoglobin-styrene oxide adducts in blood have been studied as a molecular biomarker of worker exposed to styrene. Determination of protein-styrene oxide adducts in different biological samples with modified Raney-Ni procedure is described in this paper. The following biological samples have been investigated: fresh rat blood reacted with styrene oxide in vitro: rat blood reacted with styrene or styrene oxide in vivo: vein blood from workers exposed to styrene in two factories. The data showed that there was a good linear dose-response relationship between reacting dose of styrene oxide or styrene and amount of protein-styrene oxide adducts in both in vitro and in vivo experiments. For human samples, a dose-response relationship between protein adducts and styrene exposure can be found in glass fiber factory, but not in piano manufacture plant.Hemoglobin-styrene oxide adducts in blood have been studied as a molecular biomarker of worker exposed to styrene. Determination of protein-styrene oxide adducts in different biological samples with modified Raney-Ni procedure is described in this paper. The following biological samples have been investigated: fresh rat blood reacted with styrene oxide in vitro: rat blood reacted with styrene or styrene oxide in vivo: vein blood from workers exposed to styrene in two factories. The data showed that there was a good linear dose-response relationship between reacting dose of styrene oxide or styrene and amount of protein-styrene oxide adducts in both in vitro and in vivo experiments. For human samples, a dose-response relationship between protein adducts and styrene exposure can be found in glass fiber factory, but not in piano manufacture plant.

关 键 词：Blood Drug dosage Glass fibers HEMOGLOBIN Manufacture OXIDES PROTEINS STYRENE 

分 类 号：X171.5[环境科学与工程—环境科学]