Cause of Irreversible Inactivation of Aminoacylase in Urea Solution  

作　　者：杨弋 周海梦 

出　　处：《Tsinghua Science and Technology》1999年第3期1528-1531,共4页清华大学学报（自然科学版（英文版）

摘　　要：Some have suggested that the refolding of denatured aminoacylase is difficult. The present study reports that aminoacylase denatured in urea concentrations more than 4?mol/L was markedly but partially unfolded and could be reactivated and refolded very rapidly. However, the extent of reactivation decreased with increasing time suggesting an irreversible inactivation occurred in a biphasic course. The fast phase of the irreversible inactivation is suggested to accompany the unfolding. Oxidation of the thiol groups exposed by unfolding may be one of the main causes of the second phase of the irreversible inactivation. Some have suggested that the refolding of denatured aminoacylase is difficult. The present study reports that aminoacylase denatured in urea concentrations more than 4?mol/L was markedly but partially unfolded and could be reactivated and refolded very rapidly. However, the extent of reactivation decreased with increasing time suggesting an irreversible inactivation occurred in a biphasic course. The fast phase of the irreversible inactivation is suggested to accompany the unfolding. Oxidation of the thiol groups exposed by unfolding may be one of the main causes of the second phase of the irreversible inactivation.

关 键 词：AMINOACYLASE UNFOLDING REFOLDING REACTIVATION UREA 

分 类 号：TQ441.41[化学工程—化学肥料工业]