Effect of Trichosanthin on Cell Cycle and Apoptosis of Murine Melanoma Cells  

作　　者：毕黎琦 李洪军 郑德明 

出　　处：《Chinese Journal of Integrative Medicine》1999年第1期63-65,共3页中国结合医学杂志（英文版）

摘　　要：Objective: To study the inhibitory effect of purified trichosanthin comPOnent on the proliferation of malignant melanoma. Methods: The effect of purified trichosanthin comPOnent on the DNA synthesis,cell cycle and cell aPOptosis of murine melanoma cells were detected by flow cytometry when cultured in vitro.Results: The significant GO/GI phase arrest was revealed by the increase of cells in GO/GI phase and decrease ofcells in S phase. The obvious apoptosis of melanoma cells was induced by purified trichosanthin comPOnent. GO/GI phase arrest was highly correlated with apoptosis (r = 0. 8705). Conclusion: The purified trichosanthincomPOnent can markedly inhibit melanoma cells by the supprepsion of DNA synthesis in S phase and cell mitosisas well as induction of cell apoptosis.Objective: To study the inhibitory effect of purified trichosanthin comPOnent on the proliferation of malignant melanoma. Methods: The effect of purified trichosanthin comPOnent on the DNA synthesis,cell cycle and cell aPOptosis of murine melanoma cells were detected by flow cytometry when cultured in vitro.Results: The significant GO/GI phase arrest was revealed by the increase of cells in GO/GI phase and decrease ofcells in S phase. The obvious apoptosis of melanoma cells was induced by purified trichosanthin comPOnent. GO/GI phase arrest was highly correlated with apoptosis (r = 0. 8705). Conclusion: The purified trichosanthincomPOnent can markedly inhibit melanoma cells by the supprepsion of DNA synthesis in S phase and cell mitosisas well as induction of cell apoptosis.

关 键 词：TRICHOSANTHIN MELANOMA APOPTOSIS cell CYCLE flowcytometry. 

分 类 号：R285[医药卫生—中药学]