Analysis of nucleotide sequence of wheat yellow mosaic virus genomic RNAs  

作　　者：于嘉林 晏立英 苏宁 侯占军 李大伟 韩成贵 杨莉莉 蔡祝南 刘仪 

出　　处：《Science China(Life Sciences)》1999年第5期554-560,共7页中国科学（生命科学英文版）

基　　金：Project supported by the National Natural Science Foundation of China (Grant No. 39470481);High-Tech. Program of China. The nucleotide sequence data reported in this paper have been submitted to DDBJ/GenBank/EMBL database with the accession number AF0

摘　　要：Wheat yellow mosaic virus (WYMV) isolate HC was used for viral cDNA synthesis and sequencing. The results show that the viral RNA1 is 7 629 nucleotides encoding a polyprotein with 2 407 amino acids, from which seven putative proteins may be produced by an autolytic cleavage processing besides the viral coat protein. The RNA2 is 3 639 nucleotides and codes for a polyprotein of 903 amino acids, which may contain two putative non-structural proteins. Although WYMV shares a similarity in genetic organization to wheat spindle streak mosaic virus (WSSMV), the identities in their nucleotide sequences or deduced amino acid sequences are as low as 70% and 75% respectively. Based on this result, it is confirmed that WYMV and WSSMV are different species within Bymovirus .Wheat yellow mosaic virus (WYMV) isolate HC was used for viral cDNA synthesis and sequencing. The results show that the viral RNA1 is 7629 nueleotides encoding a polyprotein with 2407 amino acids, from which seven putative proteins may be produced by an autolytie cleavage processing besides the viral coat protein. The RNA2 is 3639 nueleotides and codes for a polypretein of 903 amino acids, which may contain two putative non-structural proteins. Although WYMV shares a similarity in genetic organization to wheat spindle streak mosaic virus (WSSMV), the identities in their nucleotide sequences or deduced amino acid sequences are as low as 70% and 75 % respectively. Based on this result, it is confirmed that WYMV and WSSMV are different species within Bymovirus.

关 键 词：WHEAT YELLOW MOSAIC virus NUCLEOTIDE sequence HOMOLOGOUS analysis. 

分 类 号：S432[农业科学—植物病理学]