Expression of tPA, LH receptor and inhibin α, β_A subunits during follicular atresia in rats  

作　　者：严军丽 冯强 柳海珍 傅国强 刘以训 

出　　处：《Science China(Life Sciences)》1999年第6期583-590,共8页中国科学（生命科学英文版）

基　　金：Project supported by the National Natural Science Foundation of China (Grant Nos. 39770099 and 39770290);Chinese Academy of Sciences and Rockefeller Foundation/WHO HPR research project.

摘　　要：By using DNA 3'-end labeling, immunocytochemistry and mRNA in situ hybridization detection techniques , the expression of inhibin subunits and LH receptor in the granulosa cells and tissue-type plasminogen activator (tPA) in the oocytes has been studied in relation to follicular development and atresia. The results demonstrated that: (i) tPA activity in the oocytes of normal developing follicles is undetectable, and increases significantly in the follicle undergoing atresia; (ii) the production of inhibin subunits in granulosa cells is negatively correlated with the expression of oocyte tPA activity, indicating that they may be an important regulator of oocyte tPA production and follicular development; (iii) in atretic follicles, granulosa cells do not express LH receptor and inhibin subunits. It is therefore suggested that tPA may play a role in oocyte self-destruction and clearance in some of atretic follicles, and inhibin of granulosa-origin might be an inhibitory factor for the translation of tPA in the oocyte.By using DNA 3’-end labeling, immunocytochemistry and mRNA in situ hybridization detection techniques, the expression of inhibin subunits and LH receptor in the granulosa cells and tissue-type plasminogen activator (tPA) in the oocytes has been studied in relation to follicular development and atresia. The results demonstrated that: (ⅰ) tPA activity in the oocytes of normal developing follicles is undetectable, and increases significantly in the follicle undergoing atresia; (ⅱ) the production of inhibin subunits in granulosa cells is negatively correlated with the expression of oocyte tPA activity, indicating that they may be an important regulator of oocyte tPA production and follicular development; (ⅲ) in atretic follicles, granulosa cells do not express LH receptor and inhibin subunits. It is therefore suggested that tPA may play a role in oocyte self-destruction and clearance in some of atretic follicles, and inhibin of granulosa-origin might be an inhibitory factor for the translation of tPA

关 键 词：ATRESIA LH receptor inblbin SUBUNIT tissue PLASMINOGEN activator in SITU hybridization immunohistochemistry. 

分 类 号：Q492[生物学—生理学]