食管癌组织中hMSH2基因的表达  

Expression of hMSH2 gene in esophageal cancer

作　　者：张功员[1] 刘秋亮[2] 马春晓[2] 乐晓平[1] 葛秀峰[1] 丁一[1] 张钦宪[1] 

机构地区：[1]郑州大学分子医学重点实验室,河南郑州450052 [2]郑州大学第一附属医院小儿外科,河南郑州450052

出　　处：《第一军医大学学报》2004年第6期662-664,669,共4页Journal of First Military Medical University

基　　金：河南省科技攻关基金(324410033)~~

摘　　要：目的观察食管癌组织中DNA错配修复基因hMSH2的表达。方法32例食管癌患者术前均未接受放疗、化疗等其他治疗,标本切除后0.5 h内在癌灶、癌旁及正常残端各取1 cm×1 cm×1 cm大小的组织块,应用原位杂交法检测hMSH2。结果食管癌组织中hMSH2的阳性表达率为46.88%、癌旁为53.12%、正常组织为84.38%。与正常食管组织相比,食管癌和癌旁组织中hMSH2的阳性表达率均明显降低(P<0.05)。hMSH2阳性表达率与食管癌患者年龄及性别,肿瘤大小、肿瘤位置、病理类型、组织学分级、淋巴结转移、浸润深度等均无明显相关性(P>0.05)。结论hMSH2缺失是食管癌发生的早期事件。Objective To observe the expression of DNA mismatch repair gene hMSH2 mRNA in esophageal cancer tissues. Methods This study included 32 esophageal cancer patients who received no previous radiotherapy, chemotherapy or other treatments. Within 30 min following surgical removal of the tumor tissues, specimens of the tumor, the tissue adjacent to the tumor and normal tissue at the esophageal stump (1 cm×1 cm×1 cm in size for each specimen) were obtained for examining hMSH2 expression with hMSH2 ISH detection kit. Results The positivity rate of hMSH2 was 46.88% in the esophageal can-cer tissues, 53.12% in the adjacent tissues, and 84.38% in normal tissues at the esophageal stump, showing significant differ-ence of the former two tissues from the normal tissue (P<0.05). No significant correlation was noted between the positivity rate of hMSH2 and such factors as the patients' age, sex, tumor size, tumor location, pathological type, histological grade, lym-phatic metastasis or degree of tumor invasion (P>0.05). Conclusion The deletion of hMSH2 is an early event in the develop-ment of esophageal cancer.

关 键 词：食管肿瘤 DNA错配修复基因 HMSH2 癌组织 

分 类 号：R735.1[医药卫生—肿瘤]