氟桂利嗪对大鼠脑出血后脑细胞内游离钙的影响  

Effects of flunarizine on the intracellular free calcium concentration in acute intracerebral hemorrhage in rats

作　　者：李玲[1] 陈康宁[1] 陈福明[1] 

机构地区：[1]第三军医大学西南医院神经内科,重庆400038

出　　处：《第三军医大学学报》2004年第11期981-983,共3页Journal of Third Military Medical University

基　　金：第三军医大学校管基金资助项目 ( 2 0 0 2 )~~

摘　　要：目的　观察氟桂利嗪对脑出血不同时点脑细胞内游离钙的影响。方法　采用胶原酶法制备大鼠脑出血模型 ,利用Fura 2 AM法测定脑出血后各时点脑细胞 [Ca2 + ]i浓度 ,同时观察氟桂利嗪对上述指标影响。结果　①脑出血后0 5h细胞内游离Ca2 + 浓度开始增高 ,6h急剧增高 ,2 4h达高峰 ,72h开始降低 ,12 0h明显降低。②氟桂利嗪能明显降低脑出血早期Ca2 + 浓度。结论　氟桂利嗪降低Ca2 + 过量跨膜进入细胞内 ,减轻脑组织的病理损害 。Objective To observe the effects of flunarizine on the intracerebral hemorrhage (ICH) of Wistar rats at different time points. Methods An ICH rat model was established by collagenase. The concentrations of intracellular free Ca 2+ at different time points after ICH were determined by the fluorescence detector with an indicator, Fura 2/AM. The effects of flunarizine on the concentrations at different time points were also observed. Results The concentration of intracellular free Ca 2+ began to increase at 0.5 h, increased significantly at 6 h, and peaked at 24 h (about 4 folds as high as that before hemorrhage), but began to decrease at 72 h after acute intracerebral hemorrhage. Flunarizine decreased the concentration of intracellular free Ca 2+ significantly ( P <0.01). Conclusion Flunarizine can inhibit the excessive flow of calcium into the brain cell and decrease intracellular free Ca 2+ concentration in ICH rats, suggesting that flunarizine may have the protective effect on the injured brain cells after ICH.

关 键 词：氟桂利嗪 CA^2+ 实验性脑出血 FURA-2/AM 

分 类 号：R743.34[医药卫生—神经病学与精神病学]