维生素C和嘌呤碱的高效液相色谱之研究  

Study of Vitamine C and Purine Base Determination in Tea by High Performance Liquid Chromatography

作　　者：杨志洁[1] 叶建农[2] 刘彤[2] 方禹之[2] 

机构地区：[1]云南大理师范高等专科学校化学系,云南671000 [2]华东师范大学化学系,上海200062

出　　处：《大理学院学报（综合版）》1996年第1期43-46,共4页Journal of Dali University

摘　　要：本文报导了采用高效液相色谱(HPLC)对茶叶中维生素C、可可碱、茶碱和咖啡碱同时分离检测的方法。碳十八柱为分离柱,柱温40℃,检测波长230nm,甲醇一水(10:90)为流动相在12min内可全部出峰。方法操作简便,分离效果、重现性和线性范围均令人满意。A method leading to sin pltaneous separation and detection of vitamine C as well as several purine bases has been dse-eribed. Determination of these analytes can be accomplished within 12min using C18column at 40℃, methabol/H2OC(10: 90) as the mobile phase, followed with DAD detection at 230nm. This metod poddesses the advantages of easy operation, effective separation, good reproducibility, and satisfactory linear range.

关 键 词：茶叶 维生素C 可可碱 茶碱 咖啡碱 高效液相色谱 

分 类 号：O657.7[理学—分析化学]