EXPERIMENTAL　STUDY　ON　ANTITUMOR　EFFECT　OF　MONOCLONAL　ANTI－IDIOTYPIC　ANTIBODY－DNR　CONJUGATE　TO　LEUKEMIA  

作　　者：王晓林 曹熙芳 朱慧芬 苏娜 张悦 沈关心 

出　　处：《Chinese Journal of Cancer Research》1995年第1期20-23,共4页中国癌症研究（英文版）

摘　　要：In this experiment,the Dcxtran-T40(Dex-T40) served as intermidiate carrier,conjugated SM6 monoclonal antiidiotypic antibody(anti-Id-McAb) against B lymphocytic leukemia cells and chemotherapeutical drug daunorubicin(DNR) to form SM6:Dex: DNR immunoconjugate.The activity of anti-Id-McAb and DNR of the immunoconjugate and their extent of the substitution were measured,and the autitumor activity of SM6:hex:DNR was assayed in vitro.The results demonstrateil that using Dex-T40 to prepare immunoconjugate could get a higher extent of substitution of McAb and DNR and maintain the activity of McAb and drug well.The results of cytotoxic assay in vitro suggested that the immunoconjugate SM6:Dex:DNR possess a specific cytotoxic effett to target tumor cells.In this experiment,the Dcxtran-T40(Dex-T40) served as intermidiate carrier,conjugated SM6 monoclonal antiidiotypic antibody(anti-Id-McAb) against B lymphocytic leukemia cells and chemotherapeutical drug daunorubicin(DNR) to form SM6:Dex: DNR immunoconjugate.The activity of anti-Id-McAb and DNR of the immunoconjugate and their extent of the substitution were measured,and the autitumor activity of SM6:hex:DNR was assayed in vitro.The results demonstrateil that using Dex-T40 to prepare immunoconjugate could get a higher extent of substitution of McAb and DNR and maintain the activity of McAb and drug well.The results of cytotoxic assay in vitro suggested that the immunoconjugate SM6:Dex:DNR possess a specific cytotoxic effett to target tumor cells.

关 键 词：IMMUNOCONJUGATE Anti-Id-McAb Cytotoxic assay. 

分 类 号：R730.3[医药卫生—肿瘤]