Special Reaction and Polypeptide Analysis of Apple Rootstock Calli Under Iron Deficiency Stress  

作　　者：卞学渝 李港丽 苏润宇 陈正华 

机构地区：[1]Lab No.2 of Breeding,the Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091 [2]Department of Horticulture,Beijing Agricultural University,Beijing 100094,PRC [3]Institute of Genetics,Academia Sinica,Beijing 100101,PRC

出　　处：《Science China Chemistry》1994年第12期1463-1470,共8页中国科学（化学英文版）

基　　金：the National Natural Science Foundation of China.

摘　　要：The callus experiment systems derived from sterile stem segments subject to subcultures have been established for Malus baccata(L.)Borkh that is sensitive to iron deficiency stress,and for M.mi- cromalus Makino that is tolerant to iron deficiency stress.The physiological and biochemical changes under iron deficiency stress were compared between 2 species using these systems.Under iron deficiency stress, the dry and fresh weights of M.baccata callus increased,and the contents of elements P,S,Mg,K,Zn,B, etc.increased too,while its ferric iron reduction capacity did not change.Its nltramicrostructure of cells were damaged to some degree,and only the 25-kD polypeptide was synthesized when the NaHCO_3 was added into the medium.On the contrary,under iron deficiency stress the dry weight of M.micromalus callus did not change,the ferric iron reduction capacity increased;the content of P and S greatly decreased;the trans- fer cells were formed;and the polypeptide of 25,34 and 100 kD were synthesized.The results show that the different reactions of M.baccata and M.micromalus to iron deficiency conditions are determined by their different genetic backgrounds.It is feasible to study iron chlorosis under tissue culture condition,and it is possible to select the best species and new varieties tolerant to iron deficiency by using in vitro culture sys- tems.The callus experiment systems derived from sterile stem segments subject to subcultureshave been established for Malus baccata（L.）Borkh that is sensitive to iron deficiency stress,and for M.mi-cromalus Makino that is tolerant to iron deficiency stress.The physiological and biochemical changes underiron deficiency stress were compared between 2 species using these systems.Under iron deficiency stress,the dry and fresh weights of M.baccata callus increased,and the contents of elements P,S,Mg,K,Zn,B,etc.increased too,while its ferric iron reduction capacity did not change.Its nltramicrostructure of cellswere damaged to some degree,and only the 25-kD polypeptide was synthesized when the NaHCO₃ was addedinto the medium.On the contrary,under iron deficiency stress the dry weight of M.micromalus callus didnot change,the ferric iron reduction capacity increased;the content of P and S greatly decreased;the trans-fer cells were formed;and the polypeptide of 25,34 and 100 kD were synthesized.The results show thatthe different reactions of M.baccata and M.micromalus to iron deficiency conditions are determined by theirdifferent genetic backgrounds.It is feasible to study iron chlorosis under tissue culture condition,and it ispossible to select the best species and new varieties tolerant to iron deficiency by using in vitro culture sys-tems.

关 键 词：apple ROOT STOCK CALLUS iron DEFICIENCY POLYPEPTIDE analysis. 

分 类 号：S661.1[农业科学—果树学]