Genetic Transformation of Lycium barbarum L. via A. tumefaciens  

作　　者：杜立群 王慧中 黄发灿 李安生 邵启全 

机构地区：[1]Institute of Genetics, Academia Sinica, Beijing 100101, PRC [2]Institute of Genetics, Academia Sinica, Beijing 100101, PRC Present sddress:Institute of Chinese Medicine of Zhejiang Provine,Hangzhou 310004,PRC.

出　　处：《Science China Chemistry》1994年第3期286-293,共8页中国科学（化学英文版）

摘　　要：A system for transformation and regeneration of Lycium barbarum L. , an important Chinese medical plant, has been established. Young stem segments from Lycium barbarum L. were infected with Agrobacterium tumefaciens C58cl(pGV3850: :ineo1130), and the transformed calli selected from the callus induction medium containing 50 fig/ml kanamycin could regenerate buds on differentiation medium containing 25 μg/ml kanamycin. 30% of the regenerated buds were normal in morphology. The normal buds could develop into whole plantlets after they were transferred to the rooting medium to induce roots. Nopa-line detection, NPT-Ⅱ enzyme activity assay and Southern blotting hybridization indicated that the foreign genes had been integrated into the genome of Lycium barbarum L. and expressed in the plant. In the processes of experiments, it was found that (i) after the pre-processes, the explants which formed callus quickly were easy to transform ; (Ⅱ) the rate of normal regenerated plants from transgenic calli was higher than that from the untransgenic ones.A system for transformation and regeneration of Lycium barbarum L. , an important Chinese medical plant, has been established. Young stem segments from Lycium barbarum L. were infected with Agrobacterium tumefaciens C58cl(pGV3850: :ineo1130), and the transformed calli selected from the callus induction medium containing 50 fig/ml kanamycin could regenerate buds on differentiation medium containing 25 μg/ml kanamycin. 30% of the regenerated buds were normal in morphology. The normal buds could develop into whole plantlets after they were transferred to the rooting medium to induce roots. Nopa-line detection, NPT-Ⅱ enzyme activity assay and Southern blotting hybridization indicated that the foreign genes had been integrated into the genome of Lycium barbarum L. and expressed in the plant. In the processes of experiments, it was found that (i) after the pre-processes, the explants which formed callus quickly were easy to transform ; (Ⅱ) the rate of normal regenerated plants from transgenic calli was

关 键 词：LYCIUM barbarum L. AGROBACTERIUM TUMEFACIENS TI-PLASMID transformation REGENERATION system. 

分 类 号：Q943[生物学—植物学]