Phase Relationship in Phenol-Insulin Crystal Growth System  

作　　者：梁栋材 宋浪舟 万柱礼 常文瑞 

机构地区：[1]State Key Laboratory of Biomacromolecules,Institute of Biophysics,Academia Sinica,Beijing 100101,PRC

出　　处：《Science China Chemistry》1994年第8期948-954,共7页中国科学（化学英文版）

基　　金：the Foundation of Chinese Academy of Sciences;the National Natural Science Foundation of China

摘　　要：Based on the crystal growth system of rhombohedral 2Zn-insulin,the phase transition ofinsulin crystals has been investigated with the phenol concentration as an independent component.The dia-gram of the phase relationship in this crystal growth system was established,and two points of phase transi-tion were found.The transition point Ⅰ indicates the phase transition between rhombohedral 2Zn-insulin crys-tal and rhombohedral 4Zn-insulin crystal,and these two phases coexist within a narrow region of phenol con-centration (0.028%-0.029% (g/ml)).Point Ⅱ at 0.76%-0.77% (g/ml) of phenol concentration showsthe phase transition between rhombohcdral crystal and monoclinic crystals,and a new phase of monocliniccrystal (B-form monoclinic insulin crystal) has been observed.This paper reports the diagram of phase rela-tionship obtained from our experiments,and analyses and discusses the dependence of phase transition of in-sulin crystals on phenol concentration in crystal growth system.Based on the crystal growth system of rhombohedral 2Zn-insulin,the phase transition of insulin crystals has been investigated with the phenol concentration as an independent component.The dia- gram of the phase relationship in this crystal growth system was established,and two points of phase transi- tion were found.The transition point Ⅰ indicates the phase transition between rhombohedral 2Zn-insulin crys- tal and rhombohedral 4Zn-insulin crystal,and these two phases coexist within a narrow region of phenol con- centration (0.028%-0.029% (g/ml)).Point Ⅱ at 0.76%-0.77% (g/ml) of phenol concentration shows the phase transition between rhombohcdral crystal and monoclinic crystals,and a new phase of monoclinic crystal (B-form monoclinic insulin crystal) has been observed.This paper reports the diagram of phase rela- tionship obtained from our experiments,and analyses and discusses the dependence of phase transition of in- sulin crystals on phenol concentration in crystal growth system.

关 键 词：DIAGRAM of phase relationship phenol-insulin crystal structural STATES of INSULIN HEXAMER 

分 类 号：R91[医药卫生—药学]