Expression of Human βE-Globin Gene With Erythroid Enhancer in Transgenic Mice  

作　　者：刘庆辉 刘德培 丁玉珍 韦启泽 贾佩臣 陈松森 梁植权 

机构地区：[1]State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, PRC [2]Department of Medicine, PLA General Hospital, Beijing 100081, PRC.

出　　处：《Science China Chemistry》1994年第9期1113-1118,共6页中国科学（化学英文版）

基　　金：the Chinese National High Technology Development Program (863-102-17-2);Cheng Scholarship,and Star Foundation of UCSF School of Medicine

摘　　要：The human βE-globin gene from HbE(β26 Glu→Lys,G→A)homozygote,a commonhemoglobinopathy in China,was injected into the pronuclei of fertilized mouse eggs to prepare transgenicmice.A transgenic mouse bearing 16 copies of construct 5’HS2βE has characteristics of typical transgenicmice.It has been verified that the presence of erythroid enhancer 5’HS2 is necessary for the high level ex-pression of human βE-globin gene in transgenic mice,indicating that the cis-element 5’HS2 is effective for ab-normal β-globin gene as well.Although the expression level of β-globin gene was copy number-dependent asreported in previous studies,the average expression level per gene copy(12.1%)in transgenic mice bearingmany copies of 5’HS2βE(16 copies)was significantly lower than that(79.7%)in transgenic mice bearingfewer copies of the gene(2 copies).The possible mechanisms for this decrease of expression are discussed.Novel hemoglobin tetramers which presumably consist of human βE-globin chains and mouse endogenous α-globin chains have been assembled in transgenic mice.The human βE-globin gene from HbE(β26 Glu→Lys,G→A)homozygote,a common hemoglobinopathy in China,was injected into the pronuclei of fertilized mouse eggs to prepare transgenic mice.A transgenic mouse bearing 16 copies of construct 5'HS2βE has characteristics of typical transgenic mice.It has been verified that the presence of erythroid enhancer 5'HS2 is necessary for the high level ex- pression of human βE-globin gene in transgenic mice,indicating that the cis-element 5'HS2 is effective for ab- normal β-globin gene as well.Although the expression level of β-globin gene was copy number-dependent as reported in previous studies,the average expression level per gene copy(12.1%)in transgenic mice bearing many copies of 5'HS2βE(16 copies)was significantly lower than that(79.7%)in transgenic mice bearing fewer copies of the gene(2 copies).The possible mechanisms for this decrease of expression are discussed. Novel hemoglobin tetramers which presumably consist of human βE-globin chains and mouse endogenous α- globin chains have been assembled in transgenic mice.

关 键 词：βE-globin GENE TRANSGENIC MICE ERYTHROID ENHANCER 

分 类 号：Q78[生物学—分子生物学]